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Enzymes, Molecular Cloning, Reverse Transcriptase and RNA Polymerases, RT-PCR Kits
T7 RNA Polymerase
T7 RNA polymerase is a DNA-dependent RNA polymerase that catalyzes the formation of RNA in the 5′→ 3′ direction.
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Biochemical Reagents, Biochemical Reagents
TAE (Tris/Acetate/EDTA) Buffer, 10X
TAE buffer is the most commonly used buffer for DNA agarose gel electrophoresis but is also used for non-denaturing RNA agarose gel electrophoresis1. Double-stranded DNA tends to run faster in TAE…
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Enzymes, Mesophilic DNA Polymerases
Terminal deoxynucleotidyl Transferase (TdT)
Terminal deoxynucleotidyl transferase (TdT) is a template-independent DNA polymerase that catalyzes the addition of deoxynucleotides to the 3’ hydroxyl terminus of single or double stranded DNA molecules.
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Culture Plates, Molecular Cloning
Terrific Broth Agar Plates
1.2% Bacto tryptone, 2.4% yeast extract, 1.6% glycerol, 1.5% agar, 72mM K2HPO4, and 17mM KH2PO4.
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Enzymes, Protease, Protease, Protein Purification
TEV Protease
TEV (Tobacco etch virus) Protease is a highly site-specific cysteine protease that recognizes the cleavage site of Glu-Asn-Leu-Tyr-Phe-Gln-Gly and cleaves between Gln and Gly. TEV protease is a very useful…
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Enzymes, RNases
Thermostable RNase H
Thermostable RNase H degrades the RNA strand in RNA-DNA hybrids, it has an optimal activity above 65°C and can be used up to 95°C.
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Magnetic Beads, Nucleic Acid Purification
Tosyl Magnetic Beads
Puramag™ superparamagnetic beads are versatile tools for convenient and effective isolation of various biomolecules.
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Biochemical Reagents, Biochemical Reagents
Tris-Buffered Saline (TBS), 10X
Tris Buffered Saline (TBS) is an isotonic, non-toxic buffer used in some biomedical techniques to maintain pH within a relatively narrow range. TBS can shift pH relatively drastically as temperature…
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Biochemical Reagents, Biochemical Reagents
Tris-EDTA Buffer, 1X
TE Buffer is a common molecular biology buffer used for protection of DNA and RNA from degradation. The Tris buffering agent and EDTA metal chelating properties help to protect DNA…
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Nucleases, pAGO Nucleases, pAGO System
TtAGO (Tth Argonaute)
Prokaryotic Argonaute (pAGO) is the pivotal enzyme in the host defense system by mediating nucleic acid molecules.
Use coupon code “PAGO20OFF” to get 20% off for Thanksgiving sale! Deal ends Jan…