Cas12a2 Nuclease

$15.00$490.00

CRISPR-Cas systems are powerful molecular tools for mediating nucleic acids. Cas12a2, a Type V CRISPR/Cas nuclease, cleaves the target RNA following target RNA recognition by the complementary crRNA.

SKU: SKU49698 Categories: , , ,
SKU OPTIONS PriceQuantity
Cas12a2-100 Cas12a2 Nuclease(200 pmol, 200 µl ) (1 µM) $150.00
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Cas12a2-200 Cas12a2 Nuclease(1 nmol, 200 µl) (5 µM) $490.00
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CAS12A2-BUF-100 Cas12a2 Buffer (10X Cas12a2 Reaction Buffer)(200 ul) $15.00
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CAS12A2-BUF-200 Cas12a2 Buffer(10X Cas12a2 Reaction Buffer) (1 ml) $45.00
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Description

Description
CRISPR-Cas systems are powerful molecular tools for mediating nucleic acids. Cas12a2, a Type V CRISPR/Cas nuclease, cleaves the target RNA following target RNA recognition by the complementary crRNA. Cas12a2 processes its own crRNAs. The PFS requirement for Cas12a2 is 5′-GAAAG-3′. Cas12a2 can indiscriminately degrade single-stranded DNA (ssDNA), double-stranded DNA (dsDNA), and single -stranded RNA (ssRNA) as collateral activities once sensing correct crRNA-guided RNA binding. These properties enable Cas12a2 as a single-effector nuclease efficiently and sensitively for RNA detection.
SuCas12a2 from Sulfuricurvum sp. PC08-66 is a programmable nuclease guided by a 42-44 nt crRNA and can be applied in RNA detection and genome editing.

Features
Low Endotoxin Level
High Biological Activity

Application
RNA Detection, CRISPR Diagnostics, Genome Editing

Source
Cas12a2 gene from Sulfuricurvum sp. PC08-66 (SuCas12a2, UniProtKB: A0A0C2W1L1) expressed in E. coli

Purity
Greater than 95% as determined by SDS-PAGE and FPLC

Activity
For the cleavage assay: 20 µl reaction, including SuCas12a2 Enzyme, crRNA, target RNA and 1x reaction buffer, preheated at 37°C for 15 min. Then for the ssDNA probe cleavage assay as Fig.1 below, added 100 nM ssDNA 5’FAM probe, incubated at 37°C for 30 min; For the plasmid cleavage assay as Fig. 2 below, added 7 nM pUC19 plasmid, incubated at 37°C for 25 min, and stop reaction with 1 µl Proteinase K (800 U/ml).

Storage Condition
-20 °C

10 X Reaction Buffer
500 mM Tris-HCl, pH 7.9
1M NaCl
100 mM MgCl2
1 mg/ml BSA

Storage Buffer
25 mM HEPES, pH 7.2
150 mM NaCl
2 mM MgCl2
50% glycerol

Experimental Data

References
1. Oleg Dmytrenko, et. al. (2023). Nature. 613, 588 -– 594
2. Jack P. K. Bravo, et. al. (2023). Nature. 613, 582 -– 587

Additional information

OPTIONS

Cas12a2 Nuclease(200 pmol, 200 µl ) (1 µM), Cas12a2 Nuclease(1 nmol, 200 µl) (5 µM), Cas12a2 Buffer (10X Cas12a2 Reaction Buffer)(200 ul), Cas12a2 Buffer(10X Cas12a2 Reaction Buffer) (1 ml)

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