Taq DNA Ligase
$0.00 – $462.00
SKU | Volumes | Concentration | Price | Quantity | ||
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TDL-100 | 10,000 units | 40,000 U/ml | $154.00 | |||
TDL-200 | 40,000 units | 40,000 U/ml | $462.00 | |||
TDL-OEM | Any Size | Please inquire |
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Description
Description:
Taq DNA Ligase catalyzes the formation of a phosphodiester bond in duplex DNA containing adjacent 5′-phosphoryl and 3′-hydroxyl termini, using NAD+ as a cofactor.
Figure:
Figure. Comparing to another vendor’s product, the activity of MCLAB’s Taq DNA ligase was tested with BstEII-digested Lamda DNA under 45°C for 15 minutes.
Applications:
- Allele-specific gene detection using Ligase Detection Reaction and Ligase Chain Reaction
- Mutagenesis by incorporation of a phosphorylated oligonucleotide during primer extension amplification
Source:
A recombinant E. coli strain carrying the cloned Taq DNA Ligase gene
Supplied in:
10 mM Tris-HCl
50 mM KCl
1 mM Dithiothreitol
0.1 mM EDTA
0.1% TWEEN 20
50% Glycerol
pH 7.4 @ 25°C
Supplied with:
10x Taq DNA Ligase Buffer
10x Taq DNA Ligase Buffer:
200 mM Tris-HCl
250 mM Potassium Acetate
100 mM Magnesium Acetate
10 mM NAD 1
100 mM DTT
0.1% Triton® X-100
pH 7.6 @ 25°C
Unit Definition:
One unit is defined as the amount of enzyme required to give 50% ligation of the 12-base pair cohesive ends of 1 µg of BstEII-digested λ DNA in a total reaction volume of 50 µl in 15 minutes at 45°C.
Specific Activity: 400 Unit/µg
Recommended Storage Condition: -20°C
Additional information
OPTIONS | 10,000 units (40,000 U/ml), 40,000 units (40,000 U/ml), Any Size |
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Manual & Protocols
COA ( Certificate of Analysis )
MSDS & Certificates
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