All-In-One 5X Reverse Transcription Mix Kit
$112.00 – $395.00
MCLAB’s All-In-One 5X Reverse Transcription Mix Kit is optimized to synthesize the first strand cDNA for RT-PCR and RT-qPCR.
| SKU | OPTIONS | Price | Quantity | ||
|---|---|---|---|---|---|
| 5AIO-100 | 25 rxn(4ul/rxn),100 ul | $112.00 | |||
| 5AIO-200 | 100 rxn(4ul/rxn),400 ul | $395.00 |
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Description
Description
MCLAB’s All-In-One 5X Reverse Transcription Mix Kit is optimized to synthesize the first strand cDNA for RT-PCR and RT-qPCR. MCLAB’s QuantumScript™ HD Reverse Transcriptase is an engineered mutation with increased sensitivity, improved specificity, maximum thermostability and reduced RNase H activity. The optimal fist-strand cDNA synthesis temperature for this enzyme is 50°C. At higher temperatures the reverse transcription reaction improves cDNA yields for difficult RNA transcription. This Pre-mixed 5X concentrated solution includes all necessary components for the first-strand synthesis: 5X buffer, dNTPs, MgCl2, DTT, primers (optimized mixture oligo (dT) and random primers), RNase inhibitors, QuantumScript™ HD Reverse Transcriptase, It will simplify your first strand cDNA synthesis process.
Features
- Easy-to-use pre-mixed package
- High fidelity, yield and sensitivity
- Broader linear range
- Complete cDNA synthesis guaranteed
- Maximum sample volume available
- Robust and high throughput
- Works with difficult sequences
Application
RNA reverse transcription
Recommended Storage Condition
-20 °C
Shipping Condition
dry ice
Additional information
| OPTIONS | 25 rxn(4ul/rxn),100 ul, 100 rxn(4ul/rxn),400 ul |
|---|
Manual & Protocols
MSDS & Certificates
Figure 1. cDNA was synthesized in 20 µl for 30 minutes from 100 ng total RNA with different 5x All in one master mix. 1 ul of cDNA was used for qPCR, The qPCR was performed in MClab 2x HotSybr qPCR system.
