DNA Polymerase I
$0.00 – $455.00
| SKU | Volumes | Concentration | Price | Quantity | ||
|---|---|---|---|---|---|---|
| DPI-100 | 2,500 units | 10,000 U/ml | $152.00 | |||
| DPI-200 | 10,000 units | 10,000 U/ml | $455.00 | |||
| DPI-OEM | Any Size | Please inquire |
- Description
- Additional information
- Documents
- Q&A
Description
Description:
DNA Polymerase I is a mesophilic DNA polymerase that exhibits 5′ -> 3′ DNA synthesis activity in addition to both 3′ -> 5′ and 5′ -> 3′ exonuclease activities. The combination of DNA synthesis and 5′ -> 3′ nuclease characteristics enable nick-translation during DNA synthesis.
Applications:
- Nick translation of DNA to obtain probes with a high specific activity
- Second strand synthesis of cDNA
Source:
A recombinant E. coli strain carrying the PolA gene
Specific Activity: 6,850U/mg
Supplied in:
25 mM Tris-HCl
0.1 mM EDTA
1.0 mM DTTl
50% Glycerol
pH 7.4 @ 25°C
Supplied with:
10x Blue Buffer
10x Blue Buffer:
500 mM NaCl
100 mM Tris-HCl
100 mM MgCl2
10 mM DTT
pH 7.9 @ 25°C
Unit Definition:
1 unit is defined as the amount of polymerase required to convert 10 nmol of dNTPs into acid insoluble material in 30 minutes at 37°C.
Recommended Storage Condition: -20°C
Additional information
| OPTIONS | 2,500 units (10,000 U/ml), 10,000 units (10,000 U/ml), Any Size |
|---|
Manual & Protocols
COA ( Certificate of Analysis )
MSDS & Certificates
The Q&A for this product will be available soon.
