RNA Poly(A) Tailing Kit

Description

Description:
The RNA Poly(A) Tailing Kit provides highly pure and active enzyme and other reagents for quickly and easily adding a poly(A) tail to the 3′ end of any RNA. MCLAB’s newly engineered Poly(A) Polymerase uses ATPs as a substrate for template-independent addition of adenosine monophosphate to the 3´-hydroxyl termini of RNA molecules. Cloned Poly(A) Polymerase is encoded by an E. coli gene and overexpressed in the host strain.

Applications:

• Addition of a poly(A) tail to an RNA molecule or a mixture of RNA molecules in order to provide a priming site for synthesis of first-strand cDNA using a primer with poly(dT) on its 3´ end
• Addition of a poly(A) tail to RNA synthesized in vitro in order to increase the stability of the RNA and enhance its ability to be translated in vivo after transfection or microinjection into eukaryotic cells
• Synthesis of polyadenylated RNA for nucleic acid amplification or gene expression studies
• 3´-end labeling of RNA or quantifying mRNA

Source:
The gene encoding E. coli Poly(A) Polymerase is expressed in E. coli.

Supplied in:
25 mM Tris-HCl
500 mM NaCl
1 mM MgCl₂
0.1 mM DTT
0.1 mM EDTA
50% Glycerol
pH 8.0 @ 25°C

Supplied with:
5x Poly(A) Polymerase Reaction Buffer
10 mM ATP
25 mM MnCl₂

5x Poly(A) Polymerase Reaction Buffer:
100 mM Tris-HCl
pH 7.0
3.0 mM MnCl₂
0.1 mM EDTA
mM DTT
500 µg/ml Acetylated BSA
50% Glycerol

Recommended Storage Condition: -20°C

References:
1. Drummond, D.R. et al. (1985) J. Cell. Biol. 100, 1148.
2. Galili, G. et al. (1988) J. Biol. Chem. 263, 5764.
3. Lingner, J. and Keller, W. (1993) Nucleic Acids Res. 21, 2917.

Additional information

The Q&A for this product will be available soon.