Cas13d
$0.00 – $1,089.00
Adaptive immune systems from prokaryotes utilize clustered regularly interspaced short palindromic
repeats (CRISPRs) and CRISPR-associated (Cas) proteins to cleave foreign genetic material. Cas13d is part of the Type VI CRISPR-Cas system.
| SKU | Volumes | Concentration | Price | Quantity | ||
|---|---|---|---|---|---|---|
| CAS13d-100 | Cas13d | 100 μg,1 mg/ml | $219.00 | |||
| CAS13d-200 | Cas13d | 1 mg,1 mg/ml | $1,089.00 | |||
| CAS13d-300 | Cas13d | 100 mg,10 mg/ml | Please inquire | |||
| CAS13D-CAS13BUF-100 | Cas13 Buffer | 10X Cas13 Reaction Buffer,200 ul | $15.00 | |||
| CAS13D-CAS13BUF-200 | Cas13 Buffer | 10X Cas13 Reaction Buffer,1 ml | $45.00 |
- Description
- Additional information
- Documents
- Q&A
Description
Description
Adaptive immune systems from prokaryotes utilize clustered regularly interspaced short palindromic repeats (CRISPRs) and CRISPR associated (Cas) proteins to cleave foreign genetic material. CasRx, Cas13d from Ruminococcus flavefaciens XPD3002, was highly active and short, 112KD. In addition to programmable RNase activity, all Cas13d exhibit collateral activity after recognition and cleavage of a target transcript, leading to non-specific degradation of any nearby transcripts regardless of complementarity to the spacer.
Applications
• Targeted RNA knockdown
• RNA editing
• Nucleic acid detection
Source
expressed in E. coli
Organism
Ruminococcus flavefaciens XPD3002 (CasRx),
Molecule Weight: 112KD
Purity
> 95 % as determined by SDS-PAGE
Storage
-20 °C. Avoid repeated freeze-thaw cycles.
Cas13 Storage Buffer
0.3 M NaCl
20 mM Tris-HCl, pH 8.0
0.5 mM EDTA
1 mM DTT
50% Glycerol
10X Cas13 Reaction Buffer
200 mM HEPES, 50 mM MgCl2, 1 M NaCl, 1 mM EDTA, pH 6.5
10X Cas13 sherlock reaction buffer (requested)
200 mM HEPES, 90 mM MgCl2, pH 6.8
Additional information
| OPTIONS | Cas13d (100 μg,1 mg/ml), Cas13d (1 mg,1 mg/ml), Cas13d (100 mg,10 mg/ml), Cas13 Buffer (10X Cas13 Reaction Buffer,200 ul), Cas13 Buffer (10X Cas13 Reaction Buffer,1 ml) |
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