96-Well Pairwise Sequence Alignment

MCLAB’s 96-Well Pairwise Sequence Alignment Tool is a free online tool that can align up to 96 pairs of experimental/standard sequences

Calculates Tm, concentrations, and GC% for the given oligo.

MCLAB’s 96-Well Pairwise Sequence Alignment Tool is a free online tool that can align up to 96 pairs of experimental/standard sequences. Here are the steps to use it.

Step 1: Load Experimental Sequences

If you use MCLAB’s DNA sequencing services, you can load any of your sequencing results as experimental sequences by clicking the ALIGN link.

Step 2: Specify Standard Sequences

You can specify one or more standard sequences for alignment. By clicking the “BLAST Sequences” button, you can run the alignment program called bl2seq to align all sequence pairs.

Step 3: View the Summary Results

A summary page will be displayed after the alignment is done, where colors are used to tell different alignment percentages.

Step 4: View the Detailed Alignment Results

From the summary page, you can simply click any colored well position to view its details.