A Practical Guide to Sanger Sequencing for Microbial Templates: Reagents, Protocols, and Troubleshooting Tips
Summary: Sanger sequencing remains a gold standard for high-accuracy DNA analysis, especially when clarity, read length, and reliability matter most. Whether you're working with PCR products, genomic DNA, or challenging templates like GC-rich regions, having the right reagents and optimized protocols can significantly improve your results. This guide outlines key components...