MCNext™ UT DNA Sample Prep Kit
MCNext™ UT DNA Sample Prep Kit provides a fast and easy workflow to prepare up to 96 pooled, indexed paired-end libraries from low input amplicons (>400bp), microbial genomes and plasmids for the subsequent cluster generation and DNA sequencing using Illumina® NGS sequencing instruments.
Description
MCNext™ UT DNA Sample Prep Kit provides a fast and easy workflow to prepare up to 96 pooled, indexed paired-end libraries from low input amplicons (>400bp), microbial genomes and plasmids for the subsequent cluster generation and DNA sequencing using Illumina® NGS sequencing instruments.
The goal of this protocol is to fragmentate and add adapter sequences onto the template DNA with a single tube reaction to generate multiplexed single read or paired-end sequencing libraries.
Features
Fast Prep Workflow
- Complete protocol in 110 minutes
- Enzymatic fragmentation without sonication
- One-step fragmentation/tagging to save time
Low Input and High Throughput
- Only 1 ng input DNA needed
- Pre-mixed enzymes and nucleotides for multiplexed processing
- Plate-based processing compatible for up to 96 samples per kit
Higher Coverage Rate and Full Compatibility
- Directional transposome doubles the sequencing coverage
- Works on all the Illumina NGS platforms
Best Value on Market
- Most complete kit with all reagents included
- All 96 Illumina indices included
- Standardized library quantification prior to sample pooling and sequencing
