E. coli SSB
$0.00 – $453.00
SKU | Volumes | Concentration | Price | Quantity | ||
---|---|---|---|---|---|---|
ESSB-100 | 1.25 mg | 5.0 mg/ml | $142.00 | |||
ESSB-200 | 5.0 mg | 5.0 mg/ml | $453.00 | |||
ESSB-OEM | Any Size | Please inquire |
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Description
Description:
Single-Stranded DNA Binding Protein (SSB) preferentially binds single-stranded DNA, forming a tetramer of four identical 18.9 kDa subunits which protects 8-16 nucleotides, while not binding well to double-stranded DNA. In nature, SSB participates in DNA replication, recombination, and repair functions. In vitro, SSB has been found to stimulate certain DNA polymerase-mediated reactions by relaxing DNA secondary structure and enhancing enzyme processivity.
Applications:
- Effective in fluorescence polarization assays
- Eliminates pausing when sequencing through strong secondary structures
- Helps obtain longer read lengths in pyrosequencing for SNP analysis
Source:
A recombinant E. coli strain carrying the E. coli SSB gene
Supplied in:
50 mM Tris-HCl
200 mM NaCl
1.0 mM DTT
0.1 mM EDTA
50% Glycerol
pH 7.5 @ 25°C
Recommended Storage Condition: -20°C
Additional information
OPTIONS | 1.25 mg (5.0 mg/ml), 5.0 mg (5.0 mg/ml), Any Size |
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