TtAGO (Tth Argonaute)

$150.00$490.00

Prokaryotic Argonaute (pAGO) is the pivotal enzyme in the host defense system by mediating nucleic acid molecules.

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SKU: SKU496941 Categories: , ,
SKU OPTIONS PriceQuantity
TtAGO-10 200 pmol, 200 µl (1 µM) $150.00
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TtAGO-50 1 nmol, 200 µl (5 µM) $490.00
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Description

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Description:
Prokaryotic Argonaute (pAGO) is the pivotal enzyme in the host defense system by mediating nucleic acid molecules. As the DNA-guided endonuclease from eubacterium Thermus thermophilus, TtAGO protein targets cognate DNA without the restriction of a PAM (protospacer adjacent motif) or PFS (protospacer flanking site) on the target sequence, expanding the selection range of available target DNA sequence. Besides, compared with CRISPR/Cas systems, TtAGO have remarkable advantages as TtAGO endonuclease utilizes guide DNA rather than guide RNA in directing target DNA cleavage, which is more convenient for in vitro applications as guide DNA is more stable in ambient temperature, easier and cost-saving to synthesize than guide RNA. These capacities enable TtAGO as a powerful programmable DNA endonuclease directed by short guide DNAs in efficient Nucleic Acid Detection systems as well as in versatile Synthetic Biology platforms.

Features:
High Biological Activity
Highly Specific
Low Endotoxin Level

Application:
Nucleic Acid Detection, Cancer Diagnostics, Genetic Screening, Synthetic Biology

Source:
TtAgo gene from Thermus thermophilus (NCBI Reference Sequence: WP_011174533.1) expressed in E. coli

Purity:
Greater than 95% as determined by SDS-PAGE and FPLC

Activity:
Purified 370 bp PCR product dsDNA digested after 1 hour reaction at 83°C.

The reaction is performed as follow:
Reaction system total 10 µl, including 50 nM TtAGO Enzyme, 0.5 µM each of guide DNA, 100 ng purified 370 bp PCR product dsDNA as target DNA and 2ul 5x reaction buffer.

Reaction at 83°C for 1 hour, then incubate at 95°C for 10 min to terminate the reaction.

Storage Condition:
-20 °C

5X Reaction Buffer:
100 mM Tris-HCI, pH 8.8,
50 mM (NH4)2SO4,
50 mM KCl,
40 mM MgSO4,
0.5% Tween-20,
4 M Betaine

Storage Buffer:
10 mM Tris-HCl
300 mM NaCl
1 mM DTT
0.1 mM EDTA
50% Glycerol
pH 7.4 at 25°C

Experimental Data:

References:
1. Gang Sheng et. al. (2014). PNAS. 111 (2) 652-657.
2. Daan C. Swarts et. al. (2014). Nature. 507(7491):258-261.
3. Yuqing Qin et. al. (2022). Trends Biotechnol. 40(8):910-914.

Additional information

OPTIONS

200 pmol, 200 µl (1 µM), 1 nmol, 200 µl (5 µM)

Manual & Protocols

The Q&A for this product will be available soon.