| SKU | Options | Price | Quantity | ||
|---|---|---|---|---|---|
| dCAS12A-100 | dCas12a (200 pmol, 200 ul, 1 uM) | $154.00 | |||
| CAS12A-BUF-100-D | Cas12a Buffer(10X Cas12a Reaction Buffer,200 ul) | $15.00 | |||
| DCAS12A-BUF-200 | Cas12a Buffer(10X Cas12a Reaction Buffer,1 ml) | $45.00 |
- Description
- Additional information
- DOCUMENTS
Description
Description
CRISPR-Cas systems are powerful tools for mediating nucleic acids. Cas12a (also referred as Cpf1) nuclease, belongs to the Class 2, Type V CRISPR system. dCas12a from Lachnospiraceae bacterium (strain ND2006) is a catalytically inactive LbCas12a (D832A, with an Asp832→Ala substitution). The protein can be programmed with a gRNA and directed to bind at a desired sequence of DNA.
Features
Low Endotoxin Level
High Biological Activity
Application
Genome Editing, DNA Imaging
Source
dCas12a( D832A) from Lachnospiraceae bacterium (strain ND2006) expressed in E. coli
Purity
Greater than 95%
Storage Condition
-20 °C
10 X Reaction buffer
500 mM NaCl
100 mM Tris-HCl
100 mM MgCl2
1 mg/ml Bovine Serum Albumin (BSA) Ultrapure
(pH 7.9 at 25 °C)
Storage Buffer
500 mM NaCl
20 mM sodium acetate
0.1 mM EDTA
0.1 mM TCEP
50% Glycerol
(pH 6 at 25 °C)
References
Chen, J.S., et. al. (2018). Science. 360, 436-439.
Additional information
| Options | dCas12a (200 pmol, 200 ul, 1 uM), Cas12a Buffer(10X Cas12a Reaction Buffer,200 ul), Cas12a Buffer(10X Cas12a Reaction Buffer,1 ml) |
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