TAE (Tris/Acetate/EDTA) Buffer, 10X
$0.00 – $93.00
TAE buffer is the most commonly used buffer for DNA agarose gel electrophoresis but is also used for non-denaturing RNA agarose gel electrophoresis1. Double-stranded DNA tends to run faster in TAE than in other buffers but can also become exhausted during extended electrophoresis. Dilution of the concentrated TAE buffer produces a 1× TAE buffer with 40 mM Tris-acetate and 1 mM EDTA, pH 8.3. The 1× TAE buffer is used both in the agarose gel and as a running buffer.
| SKU | OPTIONS | Price | Quantity | ||
|---|---|---|---|---|---|
| TAE10-100 | 1 L | $34.00 | |||
| TAE10-200 | 4 L | $93.00 | |||
| TAE10-OEM | OEM | Please inquire |
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Description
Description:
TAE buffer is the most commonly used buffer for DNA agarose gel electrophoresis but is also used for non-denaturing RNA agarose gel electrophoresis1. Double-stranded DNA tends to run faster in TAE than in other buffers but can also become exhausted during extended electrophoresis. Dilution of the concentrated TAE buffer produces a 1× TAE buffer with 40 mM Tris-acetate and 1 mM EDTA, pH 8.3. The 1× TAE buffer is used both in the agarose gel and as a running buffer.
Application:
Casting and running buffer for agarose gel electrophoresis of nucleic acids.
Notes:
0.4M Tris base, 0.4M acetate, 10mM EDTA, pH 8.0.
Recommended Storage Condition: Room temperature
Reference:
1. Sambrook, J., et al. Molecular Cloning: A Laboratory Manual Cold Spring Harbor, , (1989) 23, 6.7
Additional information
| OPTIONS | 1 L, 4 L, OEM |
|---|
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