0.5M EDTA, pH 8.0

Description

Description:
EDTA, 0.5 M, pH 8.0, is a sterile-filtered solution of 0.5 M EDTA in H₂O treated with diethyl pyrocarbonate (DEPC). EDTA (Ethylenediaminetetraacetic acid) is a chelating agent, a general chemical, and a sequestrant. Suitable for use in molecular biology applications. EDTA is used extensively as chelator of divalent cations such as Ca²⁺. Useful in molecular biology applications to minimize metal ion contaminants. Also used to facilitate enzymatic reactions that could be inhibited by heavy metal traces. Inhibits enzymes, such as metalloproteases, that require divalent cations for activity.

Applications:

• Termination of metal dependent enzymatic reactions
• Protection of DNA against metal dependent nucleases

Molecular Weight: 358.19

Molecular Formula: C₁₀H₁₃N₂O₈Na₃

Recommended Storage Condition: Room Temperature

References:
1. Child, G.P. 1951. Science. 114: 466-467. PMID: 14892768
2. Friess, E.G., et al. 1954. Arch Biochem. 53: 311-313. PMID: 13208306
3. Sapeika, N. 1955. S. Afr. Med. J. 29: 137-139. PMID: 14358876
4. Sheppard, R.L., et al. 1997. Anal. Chem. 69: 477A-480A. PMID: 9253241
5. Lanigan, R.S. and Yamarik, T.A. 2002. Int. J. Toxicol. 21: 95-142. PMID: 12396676

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