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Enzymes, Mesophilic DNA Polymerases, Next-Generation Sequencing, NGS Enzyme & Amplification TechnologyT4 DNA Polymerase
T4 DNA Polymerase catalyzes the extension of a primed DNA template in the 5′→ 3′ direction. This enzyme exhibits a powerful 3′→ 5′ exonuclease activity; it lacks any inherent 5′→…
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CRISPR/Cas9 On-target Detection, Enzymes, Gene Editing, NucleasesT4 Endonuclease VII
Endonuclease VII is the product of gene 49 of bacteriophage T4. It has a mass of 18 kDa. T4 Endonuclease VII involves in DNA-packaging, genetic recombination and mismatch repair in…
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Enzymes, Enzymes, Lysozyme, Recombinant ProteinsT4 Lysozyme
Bacteriophage T4 Lysozyme breaks down bacterial cell walls. The enzyme attacks the peptidoglycans in the cell walls of bacteria and hydrolyzes the β-1,4 linkages between N-acetylmuramic acid and N-acetylglucosamine. Specific…
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Enzymes, Kinases
T4 Polynucleotide Kinase
T4 Polynucleotide Kinase (PNK) catalyzes the transfer and exchange of the terminal gamma phosphate of ATP to the 5′-hydroxyl terminus of double-and single-stranded DNA, RNA and nucleoside 3′-monophosphate molecules (1).
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DNA/RNA Ligases, Enzymes, Next-Generation Sequencing, NGS Enzyme & Amplification Technology
T4 RNA Ligase 1 (ssRNA Ligase)
T4 RNA Ligase 1 catalyzes the ligation of a 5′ phosphoryl-terminated nucleic acid donor to a 3′ hydroxyl-terminated nucleic acid acceptor through the formation of a 3’→5′ phosphodiester bond, with…
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DNA/RNA Ligases, Enzymes
T4 RNA Ligase 2 (dsRNA Ligase)
T4 RNA Ligase 2, also known as T4 Rnl2 (gp24.1), has both intermolecular and intramolecular RNA strand joining activity. Unlike T4 RNA Ligase 1, T4 RNA Ligase 2 is much…
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DNA/RNA Ligases, Enzymes, Next-Generation Sequencing, NGS Enzyme & Amplification Technology
T4 RNA Ligase 2 (truncated) (RNL2)
MCLAB’s truncated T4 RNA Ligase 2 was developed specifically for demanding Next-Generation RNA Sequencing applications. The truncated ligase 2 specifically ligates the adenylated 5´ end of an adapter to the…
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DNA/RNA Ligases, Enzymes, Next-Generation Sequencing, NGS Enzyme & Amplification Technology
T7 DNA Ligase
T7 DNA Ligase catalyzes the formation of a phosphodiester bond between a 5′ phosphate and a 3′ hydroxyl termini in duplex DNA. The enzyme joins blunt end and cohesive end…
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Enzymes, Mesophilic DNA Polymerases
T7 DNA Polymerase
T7 DNA Polymerase is a mesophilic, highly processive, and replicative DNA polymerase from bacteriophage T7. It is responsible for the rapid and accurate replication of the virus’ genome during its…
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Enzymes, Nucleases
T7 Exonuclease
T7 Exonuclease is similar to Lambda Exonuclease in that it catalyzes the stepwise hydrolysis of duplex DNA from the 5′ termini liberating 5′ mononucleotides. However, unlike Lambda Exonuclease, the enzyme…
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Molecular Cloning, Reverse Transcriptase and RNA Polymerases, RT-PCR Kits
T7 High Yield In Vitro Transcription Kit
Phage RNA polymerases are widely used for in vitro synthesis of RNA transcripts from DNA templates, which have a double-stranded promoter (at least 19 bases) upstream of the sequence to be…
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Enzymes, Molecular Cloning, Reverse Transcriptase and RNA Polymerases, RT-PCR KitsT7 RNA Polymerase
T7 RNA polymerase is a DNA-dependent RNA polymerase that catalyzes the formation of RNA in the 5′→ 3′ direction.
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