T7 DNA Ligase
$57.00 – $608.00
SKU | OPTIONS | Price | Quantity | ||
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T7DL-100 | 1,500 Weiss units (45,000 Weiss U/ml) | $57.00 | |||
T7DL-200 | 11,250 Weiss units (45,000 Weiss U/ml) | $203.00 | |||
T7DL-300 | 45,000 Weiss units (45,000 Weiss U/ml) | $608.00 |
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Description
Description:
T7 DNA Ligase catalyzes the formation of a phosphodiester bond between a 5′-phosphate and a 3′-hydroxyl termini in duplex DNA. The enzyme joins blunt ends and cohesive ends termini as well as repair single-stranded nicks in duplex DNA.
Applications:
- Joining of Okazaki fragments during replication
- Completing short-patch DNA synthesis occurring in DNA repair process
Source:
A recombinant E. coli strain carrying the T7 DNA Ligase gene
Supplied in:
20 mM Tris-HCl
300 mM NaCl
1 mM DTT
0.1 mM EDTA
50% Glycerol
pH 7.5 @ 25°C
10x Rapid Ligation Buffer:
660 mM Tris-HCl
100 mM MgCl2
10 mM DTT
10 mM ATP
75% PEG 6000
pH 7.6 @ 25°C
Unit Definition:
One Weiss unit of the enzyme catalyzes the conversion of 1 nmol of [32PPi] into Norit-adsorbable form in 20 min at 37°C. Enzyme activity is assayed in the following mixture: 66 mM Tris-HCl (pH 7.6), 6.6 mM MgCl2, 0.066 mM ATP, 10 mM DTT, 3.3 µM [32PPi].
One Weiss Unit is approximately equivalent to 67 cohesive end units (CEU). Or 1000 CEU = 15 Weiss Unit.
Specific Activity: 45 Weiss U/µg
Recommended Storage Condition: -20°C
Reference:
Doherty, A. et al. J.Biol. Chem (1996) V.271, No.19, 11083-11089.
Additional information
OPTIONS | 1,500 Weiss units (45,000 Weiss U/ml), 11,250 Weiss units (45,000 Weiss U/ml), 45,000 Weiss units (45,000 Weiss U/ml) |
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