Bovine Enterokinase (rbEK)

$0.00$906.40

Enterokinase (EK) is an enzyme produced by cells of the duodenum and involved in human digestion.

SKU: SKU49566 Categories: ,
SKU OPTIONS PriceQuantity
EK-100 10,000 U (100ul), 100ug/ml $181.28
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EK-200 100,000 U (1ml),100ug/ml $906.40
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EK-OEM Any size Please inquire
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Description

Description
Enterokinase (EK) is an enzyme produced by cells of the duodenum and involved in human digestion. It plays a role of turning trypsinogen to its active form trypsin, and indirectly activates the pancreatic digestive enzymes. Enterokinase is a specific protease that cleaves after a lysine preceded by four aspartic acids: Asp-Asp-Asp-Asp-Lys. Enterokinase will not work if the recognition site is followed by a proline.

Full Name
Enterokinase

Source
E. coli

Species
Bovine

Accession #
P98072

Molecule Weight
Recombinant Bovine Enterokinase (rbEK) as the light chain is a single glycosylated polypeptide chain with 235 a.a. and a 6-his tag. It has a predicted molecular mass of 27 kDa.

Purity
> 95 % as determined by SDS-PAGE

Endotoxin
< 1 EU per μg of the protein as determined by the LAL method

Unit Definition: One unit is defined as the amount of enzyme needed to cleave 50 μg of fusion protein in 16 hours to 95% completion at 22°C in a buffer containing 25mM Tris-HCl, pH 8.0.

Biological Activity
100 IU/μg

Storage
rbEK remains stable up to 1 year at -20°C from date of receipt. It will remain stable at 37°C for one week without losing any activity. Please avoid freeze-thaw cycles. Avoid repeated freeze-thaw cycles.

Additional information

OPTIONS

10,000 U (100ul), 100ug/ml, 100,000 U (1ml),100ug/ml, Any size

Manual & Protocols

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