T7 RNA Polymerase
$0.00 – $455.00
| SKU | OPTIONS | Price | Quantity | ||
|---|---|---|---|---|---|
| RP-100 | 25,000 units (100,000 U/ml) | $152.00 | |||
| RP-200 | 100,000 units (100,000 U/ml) | $455.00 | |||
| RP-OEM | Any Size | Please inquire |
- Description
- Additional information
- Documents
- Q&A
Description
Description:
T7 RNA Polymerase is a DNA-dependent RNA polymerase derived from the T7 bacteriophage which exhibits a high recognition specificity to the T7 promoter and terminator sequences and catalyzes the 5′ -> 3′ synthesis of RNA starting at a T7 promoter sequence(1,2).
Specific Activity: 312,500 U/mg
Applications:
- Radiolabeled RNA probe preparation
- RNA generation for in vitro translation
- RNA generation for studies of RNA structure, processing and catalysis
- Expression control via anti-sense RNA
Source:
Purified from a strain of E. coli that expresses the recombinant T7 RNA Polymerase gene.
Supplied in:
50 mM Tris-HCl
100 mM NaCl
1 mM DTT
1 mM EDTA
50% Glycerol
0.1% Triton X-100
pH 7.9 @ 25°C
Supplied with:
10x T7 RNA Polymerase Buffer
10x T7 RNA Polymerase Buffer ( 20 mM Spermidine is not included )
400 mM Tris-HCl
60 mM MgCl2
100 mM DTT
pH 7.9 @ 25°C
Unit Definition:
One unit is defined as the amount of enzyme that will incorporate 1 nmol of ATP into acid-precipitable material in 1 hour at 37°C.
Recommended Storage Condition: -20°C
References:
1. Chamberlin, M, et al. (1973) J. Biol. Chem. 248, 2235-2244, 2245-2250.
2. Chamberlin, M. et al. (1982) in The Enzymes, 3rd edition, ed. P. D. Boyer (Academic Press, New York.) 15, 87-108.
Additional information
| OPTIONS | 25,000 units (100,000 U/ml), 100,000 units (100,000 U/ml), Any Size |
|---|
Manual & Protocols
COA ( Certificate of Analysis )
MSDS & Certificates
The Q&A for this product will be available soon.
