TEV Protease
$0.00 – $595.00
TEV (Tobacco etch virus) Protease is a highly site-specific cysteine protease that recognizes the cleavage site of Glu-Asn-Leu-Tyr-Phe-Gln-Gly and cleaves between Gln and Gly. TEV protease is a very useful reagent for cleaving fusion proteins due to its high specificity and high activity rate.
| SKU | Volumes | Concentration | Price | Quantity | ||
|---|---|---|---|---|---|---|
| TEP-100 | 1 mg | 1mg/ml | $198.00 | |||
| TEP-200 | 4 mg | 1mg/ml | $595.00 | |||
| TEP-OEM | Any Size | Please inquire |
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Description
Description:
TEV (Tobacco Etch Virus) Protease is a highly site-specific cysteine protease that recognizes the cleavage site of Glu-Asn-Leu-Tyr-Phe-Gln-Gly and cleaves between Gln and Gly. TEV protease is a very useful enzyme for cleaving fusion proteins due to its high specificity and its high activity rate. TEV Protease contains a N-terminal His-tag, and it can be removed by Ni-NTA column after cleavage reaction.
Applications:
- Removal of fusion tags from recombinant proteins
- Highly dynamic and precise cleavage capabilities
- purification of proteins and peptides
Supplied With:
TEV substrate, 50 µl
10X TEV buffer
Source:
E. coli derived from Tobacco Etch Virus.
Label: His-tag
Reaction Conditions:
TEV protease is maximally active at 34°C, but its recommended to perform digests at room temperature (20°C) or 4°C. The activity of TEV protease is approximately 3-fold greater at 20°C than at 4°C.
10X TEV buffer
500 mM Tris-HCl, 1.5 M NaCl, pH 8.0
Recommended Storage Condition: -20°C
Figures:
Figure 1
Figure 2, 69.8 kDa TEV substrate protein is incubated with TurboTEV. The cleaved products are 41.2 kDa and 27.7 kDa, respectively.
Additional information
| OPTIONS | 1 mg (1mg/ml), 4 mg (1mg/ml), Any Size |
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The Q&A for this product will be available soon.
