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Enzymes, Molecular Cloning, Reverse Transcriptase and RNA Polymerases, RT-PCR KitsT7 RNA Polymerase
T7 RNA polymerase is a DNA-dependent RNA polymerase that catalyzes the formation of RNA in the 5′→ 3′ direction.
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Biochemical Reagents, Biochemical ReagentsTAE (Tris/Acetate/EDTA) Buffer, 10X
TAE buffer is the most commonly used buffer for DNA agarose gel electrophoresis but is also used for non-denaturing RNA agarose gel electrophoresis1. Double-stranded DNA tends to run faster in TAE…
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Enzymes, Mesophilic DNA Polymerases
Terminal deoxynucleotidyl Transferase (TdT)
Terminal deoxynucleotidyl transferase (TdT) is a template-independent DNA polymerase that catalyzes the addition of deoxynucleotides to the 3’ hydroxyl terminus of single or double stranded DNA molecules.
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Culture Plates, Molecular Cloning
Terrific Broth Agar Plates
1.2% Bacto tryptone, 2.4% yeast extract, 1.6% glycerol, 1.5% agar, 72mM K2HPO4, and 17mM KH2PO4.
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Enzymes, Protease, Protease, Protein Purification
TEV Protease
TEV (Tobacco etch virus) Protease is a highly site-specific cysteine protease that recognizes the cleavage site of Glu-Asn-Leu-Tyr-Phe-Gln-Gly and cleaves between Gln and Gly. TEV protease is a very useful…
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Enzymes, RNasesThermostable RNase H
Thermostable RNase H degrades the RNA strand in RNA-DNA hybrids, it has an optimal activity above 65°C and can be used up to 95°C.
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Enzymes, Nucleases
Tn5 Transposase
MCLAB’s Tn5 Transposase represents a highly active variant of the Tn5 transposase enzyme. This enzyme facilitates the random insertion of a Tn5 Transposon into target DNA in vitro.
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Next-Generation Sequencing, NGS Library Preparation Kit
Topomize Amplicon Library Prep Kit
MCLAB’s Topomize Amplicon Library Prep Kit is an innovative, fast and high quality Amplicon library construction kit for next generation sequencing.
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Next-Generation Sequencing, NGS Library Preparation Kit
Topomize DNA Library Prep Kit
MCLAB’s Topomize DNA Library Prep Kit is an innovative and fast DNA library construction kit for next generation sequencing.
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Magnetic Beads, Nucleic Acid Purification
Tosyl Magnetic Beads
Puramag™ superparamagnetic beads are versatile tools for convenient and effective isolation of various biomolecules.
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Biochemical Reagents, Biochemical Reagents
Tris-Buffered Saline (TBS), 10X
Tris Buffered Saline (TBS) is an isotonic, non-toxic buffer used in some biomedical techniques to maintain pH within a relatively narrow range. TBS can shift pH relatively drastically as temperature…
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Biochemical Reagents, Biochemical Reagents
Tris-EDTA Buffer, 1X
TE Buffer is a common molecular biology buffer used for protection of DNA and RNA from degradation. The Tris buffering agent and EDTA metal chelating properties help to protect DNA…
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