Poly(A) Polymerase, E. coli.
$0.00 – $453.00
SKU | Volumes | Concentration | Price | Quantity | ||
---|---|---|---|---|---|---|
PAP-10 | 1,000 units | 5,000U/ml | $142.00 | |||
PAP-25 | 2,000 units | 5,000U/ml | $227.00 | |||
PAP-50 | 5,000 units | 5,000U/ml | $453.00 | |||
PAP-OEM | Any Size | Please inquire |
- Description
- Additional information
- Documents
- Q&A
Description
Description:
Poly(A) Polymerase catalyzes the addition of AMP from ATP to the 3′-hydroxyl of RNA. The reaction requires Mg2+ and is template independent.
Applications:
- Labeling of RNA with ATP or cordycepin
- Poly(A) tailing of RNA for cloning or affinity purification
- Enhances translation of RNA transferred into eukaryotic cells
Source:
The gene encoding E. coli Poly(A) Polymerase expressed in E. coli
Supplied in:
25 mM Tris-HCl
500 mM NaCl
1 mM MgCl2
0.1 mM DTT
0.1 mM EDTA
50% Glycerol
pH 8.0 @ 25°C
Supplied with:
10x Poly(A) Polymerase Reaction Buffer
10x Poly(A) Polymerase Reaction Buffer:
500 mM Tris-HCl
2.5 M NaCl
100 mM MgCl2
pH 7.9 @ 25°C
Unit Definition:
1 unit is defined as the amount of enzyme that will incorporate 1 nmol of ATP into acid-insoluble material in 10 minutes at 37°C.
Recommended Storage Condition: -20°C
Additional information
OPTIONS | 1,000 units (5,000U/ml), 2,000 units (5,000U/ml), 5,000 units (5,000U/ml), Any Size |
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