| SKU | OPTIONS | Price | Quantity | ||
|---|---|---|---|---|---|
| PST-100 | 10,000 units, 20 units/ul | $222.00 | |||
| PST-200 | 10,000 units, 100 units/ul | $222.00 | |||
| PST-OEM | Any Size | Please inquire |
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Description
Recognition site: CTGCA^G
Source:
Purified from a strain of E. coli that bears the cloned Pst I gene.
Storage Buffer:
10 mM Tris-HCl (pH 7.4), 50 mM NaCl, 0.1 mM EDTA, 1 mM DTT, 50% (v/v) Triton X-100
10x Pst I Buffer
100 mM NaCl
50 mM Tris-HCl
10 mM MgCl2
100 μg/ml BSA
pH 7.9 @ 25°C
Reaction Condition:
Buffer at 37°C
Typical Ligation/Recut Assay Results:
Pst I-cleaved λ DNA is ≥95% ligated after 1 h. Of the ligated fragments, 100% are cleaved (recut) by Pst I.
Recommended Storage Condition: -20°C
Comments:
Does not cleave DNA when either the A or the 5’ C residue is N6 –methyladenine or 5-methylcytosine, respectively. Partially resistant to heat inactivation (10 minutes, 65°C). Glycerol concentrations >5% (v/v) may alter specificity.
Additional information
| OPTIONS | 10,000 units, 20 units/ul, 10,000 units, 100 units/ul, Any Size |
|---|
Manual & Protocols
COA ( Certificate of Analysis )
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