PcrA Helicase
$218.00
PcrA is an ATP-driven 3′ to 5′ helicase responsible for the unwinding of double-stranded DNA (dsDNA). PcrA binds to and unravels of dsDNA into two strands of single-stranded DNA (ssDNA).
| SKU | Volumes | Concentration | Price | Quantity | ||
|---|---|---|---|---|---|---|
| PcrAH-100 | 100 μg | 1 mg/ml | $218.00 |
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Description
Description:
PcrA is an ATP-driven 3′ to 5′ helicase responsible for the unwinding of double-stranded DNA (dsDNA). PcrA binds to and unravels of dsDNA into two strands of single-stranded DNA (ssDNA).
Applications:
Helicase-dependent DNA isothermal amplification
Source:
expressed in E. coli
Organism:
Geobacillus stearothermophilus
Storage:
-20°C
Storage Buffer:
600 mM NaCl, 50 mM Tris pH 7.6, 50% glycerol
10X Reaction Buffer:
500 mM Potassium acetate, 200 mM Tris-acetate, 100 mM Magnesium acetate, 1 mg/ml BSA, pH 7.9
Experiment Result:
Helicase-dependent DNA isothermal amplification reaction (65 °C, 1 hour) using PcrA helicase for cloning 667 bp and 871 bp DNA fragments. Template of the reaction is 5 ng DNA.
References:
1. M. Dittrich, et al., (2006) Structure 14, 1345–1353
2. M. Gavrilov, et al., (2022) Nature Communications 13:6312
Additional information
| OPTIONS | 100 μg ( 1 mg/ml), 000 |
|---|
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