BL21(DE3) Competent E. coli
$125.00 – $363.00
SKU | OPTIONS | Price | Quantity | ||
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BS-100 | 10x100ul (10 tubes), >1x10⁸ | $125.00 | |||
BS-196 | 96x50ul (96 well PCR plate), >1x10⁸ | $363.00 | |||
BS-196T | 96 x 50µl (Bendable tear-off 96 well PCR plate) | $363.00 |
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Description
Description:
High yields, high efficiency, and no heat shock necessary.
Application:
- Ideal for expressing proteins in E. coli.
- BL21(DE3) cells carry the lambda DE3 lysogen.
Recommended storage condition:
This product should be stored at -80°C. Thaw on ice only before use. Do not re-freeze.
Recommended reaction conditions:
- Mix DNA with competent cells
- Let stand on bench for 5 minutes
- Direct plate
Genotype:
F– ompT gal dcm lon hsdSB(rB- mB-) λ(DE3 [lacI lacUV5-T7 gene 1 ind1 sam7 nin5])
Reference:
Miroux B, Walker JE. J. Mol. Biol. (1996) 260, 289-298.
Competent Cell Type:
Chemically
Transformation Efficiency (cfu/μg):
>1×108
Blue-Whiet Screening:
No
Strain:
B
Reduces Recombination:
No
Cloning Methylated DNA:
No
Improves Plasmid Quality:
No
Preparing Unmethylated DNA:
Not suitable
T1 Phage Resistant:
No
RecA Deficient:
No
Additional information
OPTIONS | 10x100ul (10 tubes), >1×10⁸, 96x50ul (96 well PCR plate), >1×10⁸, 96 x 50µl (Bendable tear-off 96 well PCR plate) |
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Manual & Protocols
COA ( Certificate of Analysis )
MSDS & Certificates
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