BL21 Competent E. coli

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BL21(DE3) Competent E. coli

$125.00 – $363.00

Chemically competent E. coli cells suitable for transformation and protein expression. This strain does not express the T7 RNA polymerase.
SKU: BL-100 Categories: ,
SKU OPTIONS PriceQuantity
BL21-100 10x100ul (10 tubes), >1x10⁸ $125.00
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BL21-196 96x50ul (96-tube rack), >1x10⁸ $363.00
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Description

Description:
Chemically competent E. coli cells suitable for transformation and protein expression. This strain does not express the T7 RNA polymerase.

Application:

  • Ideal for expressing proteins in E. coli

Recommended storage condition:
This product should be stored at -80°C. Thaw on ice only before use. Do not re-freeze.

Recommended reaction conditions:

  • Mix DNA with competent cells
  • Let stand on bench for 5 minutes
  • Direct plate

Genotype:
E. coli B F- dcm ompT hsdS(rB- mB-) gal [malB+]K-12(λS)

Reference:
Miroux B, Walker JE. J. Mol. Biol. (1996) 260, 289-298.

Competent Cell Type:
Chemically

Transformation Efficiency (cfu/μg):
>1×108

Blue-Whiet Screening:
No

Strain:
B

Reduces Recombination:
No

Cloning Methylated DNA:
No

Improves Plasmid Quality:
No

Preparing Unmethylated DNA:
Not suitable

T1 Phage Resistant:
No

RecA Deficient:
No

Additional information

OPTIONS

10x100ul (10 tubes), >1×10⁸, 96x50ul (96-tube rack), >1×10⁸

 

COA ( Certificate of Analysis )

 

MSDS & Certificates

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