T4 DNA Ligase
$0.00 – $280.00
| SKU | Volumes | Concentration | Price | Quantity | ||
|---|---|---|---|---|---|---|
| TL-100 | 40,000 Unit | 400,000 u/ml | $70.00 | |||
| TL-200 | 200,000 Unit | 400,000 u/ml | $280.00 | |||
| TL-300 | 40,000 Unit | 2,000,000 u/ml | $70.00 | |||
| TL-400 | 200,000 Unit | 2,000,000 u/ml | $280.00 | |||
| TL-OEM | Any Size | Please inquire |
- Description
- Additional information
- Documents
- Q&A
Description
Description:
T4 DNA Ligase catalyzes the formation of a phosphodiester bond between juxtaposed 5′-phosphate and 3′-hydroxyl termini in duplex DNA or RNA using ATP as a cofactor.
Figure. Ligation of λ DNA/Hind III fragments analyzed by agarose gel electrophoresis.
Performances of T4 DNA ligase from MCLAB and another supplier were compared within identical reaction mixture at 37°C for 30 minutes. Paralleled negative control was run without ligase.
Applications:
- Cloning of restriction fragments
- Joining linkers and adapters to blunt-ended DNA
Source:
A recombinant E. coli strain carrying the cloned T4 DNA Ligase gene.
Supplied In:
10 mM Tris-HCl
50 mM KCl
1 mM DTT
0.1 mM EDTA
50% Glycerol
pH 7.4 @ 25°C
Supplied With:
10x T4 DNA Ligase Buffer
10x T4 DNA Ligase Buffer:
500mM Tris-HCl
100 mM MgCl2
50 mM DTT
10 mM ATP
pH 7.6 @ 25°C
Unit Definition
One unit is defined as the amount of enzyme required to give 50% ligation of HindIII fragments of λ DNA (5´ DNA termini concentration of 0.12 µM, 300- µg/ml) in a total reaction volume of 20 μl in 30 minutes at 16°C in 1X T4 DNA Ligase Reaction Buffer.
Specific Activity: 300U/µg
Recommended Storage Condition: -20°C
Reference:
1. Engler, M.J. and Richardson, C.C. (1982) P.D. Boyer (Eds.), The Enzymes, 5, pp. 3. San Diego: Academic Press.
Additional information
| OPTIONS | 40,000 Unit(400,000 u/ml), 200,000 Unit(400,000 u/ml), 40,000 Unit(2,000,000 u/ml), 200,000 Unit(2,000,000 u/ml), Any Size |
|---|
Manual & Protocols
MSDS & Certificates
The Q&A for this product will be available soon.
