RNase H, Bst

$0.00$521.18

RNase H is an endoribonuclease which degrades the RNA strand of RNA/DNA hybrid molecules.

SKU: RNHB Categories: ,
SKU OPTIONS PriceQuantity
RNHB-100 2,500U (10,000u/ml) $158.62
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RNHB-200 10,000U (10,000u/ml) $521.18
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RNHB-OEM Any Size Please inquire
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Description

Description
RNase H is an endoribonuclease which degrades the RNA strand of RNA/DNA hybrid molecules. RNase H digestion produces ribonucleotide molecules with 5′-phosphate and 3′-hydroxyl termini. RNAse H is nearly inactive against single or double-stranded RNA molecules.
RNase H from the thermophilic bacterium Bacillus stearothermophilus is thermostable, has optimal activity above 65°C and making it useful for a broad range of applications.

Applications

  • Removal of poly(A) tails of mRNA hybridized to poly(dT)
  • Removal of mRNA during second strand cDNA synthesis

Source:
A recombinant E. coli strain carrying the RNAse H gene from the thermophilic bacterium Bacillus stearothermophilus

Supplied in:
20 mM Tris-HCl
100 mM KCl
10 mM MgCl2
0.1 mM EDTA
0.1 mM DTT
50% Glycerol
pH 7.9 @ 25°C

Supplied with:
10x RNAse H Buffer

10x RNAse H Buffer:
500 mM Tris-HCl
750 mM KCl
30 mM MgCl2
100 mM DTT
pH 8.3 @ 25°C

Unit Definition:
1 unit is defined as the amount of enzyme that will hydrolyze 1 nmol of RNA from an 3H-labeled DNA:RNA hybrid molecule into acid-soluble material in 20 minutes at 37°C.

Recommended Storage Condition: -20°C

Additional information

OPTIONS

2,500U (10,000u/ml), 10,000U (10,000u/ml), Any Size

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