2X Ori-Master Mix
$225.00 – $5,438.00
The 2X Ori-Master Mix is a premixed, ready-to-use solution containing a high-fidelity DNA polymerase, ori specific primers, dNTPs, and MgCl2.
| SKU | OPTIONS | Price | Quantity | ||
|---|---|---|---|---|---|
| OMM-100 | 2.5 ml for 200 rxn | $225.00 | |||
| OMM-200 | 10 ml for 800 rxn | $676.00 | |||
| OMM-300 | 100 ml for 8000 rxn | $5,438.00 |
- Description
- Additional information
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- Q&A
Description
Description:
The 2X Ori-Master Mix is a premixed, ready-to-use solution containing a high-fidelity DNA polymerase, ori specific primers, dNTPs, and MgCl2. The Master Mix can produce linear copies of bacterial plasmids from a single colony. The reaction mixture and conditions are optimized regardless of the plasmid copy number. Amplified products can be directly sequenced without the need for purification.
Applications:
- Colony PCR
- DNA sequencing
- Restriction Digestion
Features:
- Fast reaction : less than 2hrs from a colony to its DNA products, comparing to 16-24 hours for a miniprep process
- Good yield: a 25 μl reaction yields up to 1 μg DNA
- Easy to use: Ori-specific primers are included and the PCR protocol is optimized. Just add the template and water.
- Long amplification fragments: up to 10 Kb
- High throughput capability: 96 or 384 well plate compatible
Experimental Data :
Figure. Colony PCR Using 2X Ori-Master Mix
M:1 Kb DNA ladder (NEB). 1&2:PCR product of ~4Kb plasmids.
Additional information
| OPTIONS | 2.5 ml for 200 rxn, 10 ml for 800 rxn, 100 ml for 8000 rxn |
|---|
The Q&A for this product will be available soon.
