2X HotStart PCR Master Mix


Designed for routine PCR, genotyping and mutiplex (multiple pairs of primers) PCR. Better than or as good as ABI’s AmpliTaq Gold, but priced 25% less!

Friendly Reminder : This product is a replacement of an equivalent product : 2X Universal Taq Master Mix.

SKU: HMM Categories: , , ,
SKU OPTIONS PriceQuantity
HMM-100 100 reactions (50 ul vol) $68.00
HMM-300 500 reactions (50ul vol) $272.00
HMM-1000 1000 reactions (50ul vol) $400.00
HMM-5000 5,000 reactions (50 ul vol) Please inquire
HMM-25k 25,000 Reactions (50 ul vol) Please inquire
HMM-OEM 2X HotStart PCR Master Mix (Oem) Please inquire


2x HotStart PCR Master Mix is a premixed 2x concentrated solution of HoTaq DNA Polymerase, reaction buffer, MgCl2 and dNTPs. The DNA template and primers are simply added for PCR reactions. The consistency and efficiency of routine PCR amplifications are optimized.

MCLAB’s HoTaq is modified from Taq DNA polymerase. The HoTaq is inactive at room temperature, but becomes active after 10 minutes at 95ºC.

Here is the result of comparing MCLAB’s HoTaq with some other leading brands.


  • Hot-start to keep background low
  • Solves the primer-dimer problem
  • All handlings can be done at room temperature
  • Easy calculation
  • Taq DNA Polymerase in ready-to-use mixture
  • Low contamination risk


  • Routine PCR
  • Genotyping
  • Multiplex (multiple pairs of primers) PCR

Please follow the instructions for each PCR reaction (20µl final volume; can be done at room temperature):
1. Mix DNA template and primers
2. Add H2O to bring the total volume to 10µl
3. Add 10µl 2x HotStart Taq PCR Master Mix
4. Set the first PCR step to 95ºC for 10 minutes to activate the HoTaq
5. Continue PCR as usual

Recommended Storage Condition: -20ºC

Shipping condition: Dry ice

Additional information


100 reactions (50 ul vol), 500 reactions (50ul vol), 1000 reactions (50ul vol), 5,000 reactions (50 ul vol), 25,000 Reactions (50 ul vol), 2X HotStart PCR Master Mix (Oem)


COA ( Certificate of Analysis )


The Q&A for this product will be available soon.