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MC1061 Competent E. coli

Placeholder MC Cloning 5’/3’-RACE Kit
Placeholder Poly(A) Polymerase, E. coli.

$125.00 – $363.00

Chemically competent E. coli cells with high stability suitable for high efficiency transformation.

SKU: SKU49463 Categories: ,
SKU OPTIONS PriceQuantity
MC1061-100 10x100 ul (10 tubes), >1.0x108 $125.00
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MC1061-196 96x50 ul (96 well PCR plate), >1.0x108 $363.00
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MC1061-196T 96 x 50µl (Bendable tear-off 96 well PCR plate) $363.00
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Bulk Orders Add selected to cart ✓

Description

Description:

  • Chemically competent E. coli cells with high stability suitable for high efficiency transformation  For research use only.
  • Parent of DH10B/TOP10 and derived strains

Applications:

  • For cloning, sub-cloning and phage display, but cannot be used for reinfection
  • Transform efficiency is >1.0×108 cfu/µg with pUC19 control DNA

Genotype:
F- Δ(ara-leu)7697 [araD139]B/r Δ(codB–lacI)3 galK16 galE15 λ- e14- mcrA0 relA1 rpsL150(StrR) spoT1 mcrB1 hsdR2(r-m+)

Recommended storage condition:
This product should be stored at -80°C. Thaw on ice only before use. Do not re-freeze

Competent Cell Type:
Chemically

Transformation Efficiency (cfu/μg):
>1.0×108 cfu/µg with pUC19 control DNA

Blue-White Screening:
Yes, IPTG induction required

Strain:
K12

Reduces Recombination:
Yes

Cloning Methylated DNA:
Yes

Improves Plasmid Quality:
Yes

Preparing Unmethylated DNA:
Not suitable

T1 Phage Resistant:
No

Additional information

OPTIONS

10×100 ul (10 tubes), >1.0×108, 96×50 ul (96 well PCR plate), >1.0×108, 96 x 50µl (Bendable tear-off 96 well PCR plate)

Manual & Protocols

The Q&A for this product will be available soon.