MC Cloning 5’/3’-RACE Kit
$0.00 – $680.00
The MC Cloning 5’/3′ RACE Kit provides a convenient method for performing both 5′ and 3′ rapid amplifications of cDNA ends (RACE) with optimized primers for cloning any gene of interest.
| SKU | OPTIONS | Price | Quantity | ||
|---|---|---|---|---|---|
| MCC5-100 | 10 reactions | $453.00 | |||
| MCC5-200 | 20 reactions | $680.00 | |||
| MCC5-OEM | Any size | Please inquire |
- Description
- Additional information
- Documents
- Q&A
Description
Description:
The MC Cloning 5’/3′ RACE Kit provides a convenient method for performing both 5′ and 3′ rapid amplifications of cDNA ends (RACE) with optimized primers for cloning any gene of interest. MCLAB’s SmartRT reverse transcriptase is an engineered MMLV RT with increased thermostability, improved cDNA synthesis ability, and a reduced RNase H activity. The enzyme also has a terminal transferase activity. This tailing activity of the SmartRT reverse transcriptase allows to synthesize complete cDNA by SMART (Switching Mechanism At 5′ end of RNA Transcript) cDNA synthesis technology. With a few additional nucleotides added to the 3′ end of the first-strand cDNA, 5′ SMART universal oligo, containing terminal complementation to the nucleotides at 3′ end of the first-strand cDNA, can be annealed to first-strand cDNA tail and serves as an extended template for RT. The switch of template from mRNA to 5′ SMART universal oligo produces a complete cDNA copy of transcript RNA with 5′ SMART universal oligo at the end. The cDNA transcript from SmartRT can be used directly in 5′ and 3′ RACE with MCLAB’s I-5™ 2X High-Fidelity Master Mix.
Features:
• Specific full length cDNA enrichment technique
• Optimized primer set for full length cDNA sequencing
• Simplified protocol without 5′ end adaptor-ligation step
Applications:
• Generating full-length cDNAs
• Structural and expression studies of RNA molecules
• Isolation and characterization of 5′ or 3′ ends from low-copy RNA messages
• Amplification and further cloning of rare mRNAs
• Use in conjunction with exon-trapping methods
Components:
1.
| Components | Cap Color | Volume (10 rxn) | Volume (20 rxn) |
| Oligo dT Primer | Orange | 10 µl | 20 µl |
| 5X RT Reaction Buffer | Green | 40 µl | 80 µl |
| dNTP (10 mM) | Yellow | 10 µl | 20 µl |
| DTT (100 mM) | Yellow | 5 µl | 10 µl |
| SmartRT™ Reverse Transcriptase | Clear | 20 µl | 40 µl |
| 5′ PCR Universal Primer (10µM) | Red | 10 µl | 20 µl |
| 3′ Universal Primer (10µM) | Red | 10 µl | 20 µl |
| I-5™ 2X High-Fidelity Master Mix | Purple | 100 µl | 200 µl |
| RNAse Inhibitor | Blue | 5 µl | 10 µl |
Storage Condition:
-20 °C
2. 5′ SMART Universal Oligo, 10 µl/20 µl. Storage Condition: -80 °C
Additional information
| OPTIONS | 10 reactions, 20 reactions, Any size |
|---|
Manual & Protocols
The Q&A for this product will be available soon.
