| SKU | Volumes | Concentration | Price | Quantity | ||
|---|---|---|---|---|---|---|
| ECOR-100 | 50,000 units | 200 units/ul | $178.00 | |||
| ECOR-200 | 50,000 units | 1,000 units/ul | $178.00 | |||
| ECOR-OEM | Any Size | Please inquire |
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Description
Recognition site: G^AATTC
Synonyms:
EcoRI, FunII
Source:
Purified from a strain of E. coli that bears the cloned EcoR I gene.
Supplied In:
50 mM Tris-HCL(pH 7.2)
300 mM NaCl
5 mM EGTA
0.5 mM EDTA
5 mM 2-mercaptoethanol
0.5 mg/ml BSA
50% (v/v) Glycerol
0.2% (w/v) Triton X-100
Reaction Condition:
Buffer at 37ºC
10x EcoR I Buffer
1M Tris-HCl
500 mM NaCl
100 mM MgCl2
0.25% Triton® X-100
pH 7.5 @ 25°C
Typical Ligation/Recut Assay Results:
EcoR I –cleaved λ DNA is ≥95% ligated after 1 h. Of the ligated fragments, 100% are cleaved (recut) by EcoR I.
Recommended Storage Condition: -20 ºC.
Comments:
Does not cleave DNA when either the 3′ A or the C residue is N6-methyladenine or 5-methylcytosine, respectively. Partially resistant to heat inactivation (10 minutes, 65 ºC). Glycerol concentrations >5% (v/v), low-salt, or high-pH conditions may alter specificity.
Additional information
| OPTIONS | 50,000 units (200 units/ul), 50,000 units (1,000 units/ul), Any Size |
|---|
Manual & Protocols
COA ( Certificate of Analysis )
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he Q&A for this product will be available soon.
