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Enzymes, Nucleases
T7 Exonuclease
T7 Exonuclease is similar to Lambda Exonuclease in that it catalyzes the stepwise hydrolysis of duplex DNA from the 5′ termini liberating 5′ mononucleotides. However, unlike Lambda Exonuclease, the enzyme…
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Molecular Cloning, Reverse Transcriptase and RNA Polymerases, RT-PCR Kits
T7 High Yield In Vitro Transcription Kit
Phage RNA polymerases are widely used for in vitro synthesis of RNA transcripts from DNA templates, which have a double-stranded promoter (at least 19 bases) upstream of the sequence to be…
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Enzymes, Molecular Cloning, Reverse Transcriptase and RNA Polymerases, RT-PCR Kits
T7 RNA Polymerase
T7 RNA polymerase is a DNA-dependent RNA polymerase that catalyzes the formation of RNA in the 5′→ 3′ direction.
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Biochemical Reagents, Biochemical Reagents
TAE (Tris/Acetate/EDTA) Buffer, 10X
TAE buffer is the most commonly used buffer for DNA agarose gel electrophoresis but is also used for non-denaturing RNA agarose gel electrophoresis1. Double-stranded DNA tends to run faster in TAE…
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Enzymes, Mesophilic DNA Polymerases
Terminal deoxynucleotidyl Transferase (TdT)
Terminal deoxynucleotidyl transferase (TdT) is a template-independent DNA polymerase that catalyzes the addition of deoxynucleotides to the 3’ hydroxyl terminus of single or double stranded DNA molecules.
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Culture Plates, Molecular Cloning
Terrific Broth Agar Plates
1.2% Bacto tryptone, 2.4% yeast extract, 1.6% glycerol, 1.5% agar, 72mM K2HPO4, and 17mM KH2PO4.
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Enzymes, Protease, Protease, Protein Purification
TEV Protease
TEV (Tobacco etch virus) Protease is a highly site-specific cysteine protease that recognizes the cleavage site of Glu-Asn-Leu-Tyr-Phe-Gln-Gly and cleaves between Gln and Gly. TEV protease is a very useful…
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Enzymes, RNases
Thermostable RNase H
Thermostable RNase H degrades the RNA strand in RNA-DNA hybrids, it has an optimal activity above 65°C and can be used up to 95°C.
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Enzymes, Nucleases
Tn5 Transposase
MCLAB’s Tn5 Transposase represents a highly active variant of the Tn5 transposase enzyme. This enzyme facilitates the random insertion of a Tn5 Transposon into target DNA in vitro.
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Magnetic Beads, Nucleic Acid Purification
Tosyl Magnetic Beads
Puramag™ superparamagnetic beads are versatile tools for convenient and effective isolation of various biomolecules.