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Enzymes, Mesophilic DNA Polymerases
DNA Polymerase I
DNA Polymerase I is a DNA-dependent DNA polymerase. The enzyme catalyzes 5′ -> 3′ synthesis of DNA and exhibits 3′ -> 5’ exonuclease proofreading activity.
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Enzymes, Thermophilic DNA Polymerases
DNA polymerase, thermotoga neapolitana
DNA polymerase from Thermotoga neapolitana was identified[1] as an enzyme that is able to withstand the protein-denaturing conditions (high temperature) required during PCR[2].
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Chaperon Proteins and Disulfide Bond Isomerase, Chaperon Proteins and Disulfide Bond Isomerase, Enzymes, Protein Purification
DnaK (HSP70)
DnaK is the prokaryotic analogue of eukaryotic Hsp70, a member of the heat shock proteins. It assists the protein assembly, folding, and translocation.
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DNA Repair Proteins, Enzymes
Endonuclease III (Nth)
Endonuclease III (Nth) protein from E. coli acts as both N-glycosylase and a AP-lyase. The N-glycosylase activity releases damaged pyrimidines from double-stranded DNA, generating a basic (AP site).
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DNA Repair Proteins, Enzymes
Endonuclease IV, E. coli
A class II apurinic/apyrimidic (AP) enzyme that cleaves 5’ to an AP site by hydrolysis leaving a hydroxyl group at the 3´ terminus and a deoxyribose 5´-phosphate at the 5´…
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DNA Repair Proteins, Enzymes
Endonuclease V, E. coli
Endonuclease V, (Endo V) is a 3′-endonuclease involved in DNA repair, which initiates removal of deaminated bases from damaged DNA, including uracil, hypoxanthine, and xanthine.
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DNA Repair Proteins, Enzymes
Endonuclease VIII, E. coli
Endonuclease VIII from E. coli acts as both an N-glycosylase and an AP-lyase. The N-glycosylase activity releases damaged pyrimidines from double-stranded DNA, generating an apurinic (AP site).
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Enzymes, Nucleases
Exonuclease I (E. coli)
Exonuclease I cleaves single-stranded DNA in the 3′→ 5′ direction, releasing 5′ -mono/di-nucleotides and leaving double-stranded DNA molecules and the 5′-terminus intact.
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Enzymes, Nucleases
Exonuclease III (E. coli)
Exonuclease III is a 3′→ 5′ exonuclease which acts by digesting one strand of a dsDNA duplex at a time or digesting the RNA strand of an RNA-DNA heteroduplex (1).
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Enzymes, Nucleases
Exonuclease VII
Exonuclease VII, (Exo VII) derived from E. coli, cleaves single-stranded DNA (ssDNA) from both 5´→3´ and 3´→5´ directions.