T7 Exonuclease

$0.00$347.00

T7 Exonuclease is similar to Lambda Exonuclease in that it catalyzes the stepwise hydrolysis of duplex DNA from the 5′ termini liberating 5′ mononucleotides. However, unlike Lambda Exonuclease, the enzyme has low processivity and it removes both 5’-hydroxyl and 5’-phosphoryl termini.
SKU: T7G6E Categories: ,
SKU OPTIONS PriceQuantity
T7G6E-100 2,500 units (10,000 U/ml) $116.00
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T7G6E-200 10,000 units (10,000 U/ml) $347.00
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T7G6E-OEM Any Size Please inquire
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Description

Description:
T7 Exonuclease is similar to Lambda Exonuclease in that it catalyzes the stepwise hydrolysis of duplex DNA from the 5′-termini, liberating 5′-mononucleotides. However, unlike Lambda Exonuclease, the enzyme has low processivity and it removes both 5′-hydroxyl and 5′-phosphoryl termini. T7 Exonuclease hydrolyzes duplex DNA non-processively in the 5′ -> 3′ direction from both 5′-phosphoryl or 5′-hydroxyl nucleotides by liberating oligonucleotides, as well as mononucleotides, until about 50% of the DNA is acid soluble.

Source:
Purified from an E. coli strain containing a TYB12 intein fusion.

Storage Conditions
10 mM Tris-HCl
5 mM DTT
0.1 mM EDTA
50% Glycerol
pH 8.0 @ 25°C

Applications:

  • Controlled stepwise digestion of double-stranded DNA from the 5′-termini
  • Generating ssDNA templates for sequencing via the chain-termination method

Supplied with:
10X T7 Exonuclease Buffer

10X T7 Exonuclease Buffer:
500 mM Potassium Acetate
200 mM Tris-acetate
100 mM Magnesium Acetate
10 mM DTT
pH 7.9 @ 25°C

Unit Definition:
One unit is the amount of enzyme required to release 1 nmol of acid soluble nucleotide in 15 min at 37°C under standard assay conditions.

Recommended Storage Condition: -20°C

References:
1. Kerr, C. and Sadowski, P. D. (1972) J. Biol. Chem. 247, 311-318.
2. Thomas, K. R. and Olivera, B. M. (1978) J. Biol. Chem. 253, 424-429.
3. Ausubel, F. M., Brent, R., Kingston, R. E., Moore, D. D., Seidman, J. G., Smith, J. A. and Struhl, K., (1987) Current Protocols in Molecular Biology (John Wiley and Sons, Inc.
4. Shon, M., Germino, J. and Bastia, D. (1982) J. Biol. Chem. 257, 13823-13827.
5. Nikiforov, T. T., Rendle, R. B., Goelet, P., Rogers, Y. H., Kotewicz,
6. M. L., Anderson, S., Trainor, G. L. and Knapp, M. R. (1994) Nucl. Acids Res 22, (20), 4167-4175.
7. Kornberg, A. and Baker, T. (1991) DNA Replication, Second Edition, 591.

Additional information

OPTIONS

2,500 units (10,000 U/ml), 10,000 units (10,000 U/ml), Any Size

Manual & Protocols

 

COA ( Certificate of Analysis )

 

MSDS & Certificates

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