Dh5-Alpha Competent E. coli
$125.00 – $3,966.00
| SKU | OPTIONS | Price | Quantity | ||
|---|---|---|---|---|---|
| DA-100 | 20x50ul (20 tubes), >1x10⁹ | $125.00 | |||
| DA-196 | 96x50ul (96 well PCR plate), >1x10⁹ | $317.00 | |||
| DA-196T | 96 x 50µl (Bendable tear-off 96 well PCR plate) | $317.00 | |||
| DA-144A* | One-year pre-payment: 144x10x100μl or 15 PCR plates (DA-196 Kit). Shipping and handling charges apply. >1x10⁹. | $3,966.00 |
- Description
- Additional information
- Documents
- Q&A
Description
Description:
DH5a™ is the most frequently used E. coli strain for routine cloning applications. In addition to supporting blue/white screening recA1 and endA1 mutations in DH5a™ increase insert stability and improve the quality of plasmid DNA prepared from minipreps.
Application:
- Highest transformation efficiency
- General cloning
- Blue-white selection
- Plasmid isolation
Recommended storage condition:
This product should be stored at -80°C. Thaw on ice only before use. Do not re-freeze.
Genotype:
F- 80dlacZ M15 (lacZYA-argF) U169 recA1 endA1hsdR17(rk-, mk+) phoAsupE44 -thi-1 gyrA96 relA1
Reference:
Woodcock, D.M., Crowther, D.M., Doherty, J., Jefferson,S., DeCruz, E., Noyer-Weidner, M., Smith, S.S., Michael, M.Z., and Graham, M.W., Nucl. Acids Res. (1989) 17, 3469-3478
Competent Cell Type: Chemically
Transformation Efficiency (cfu/μg):
>1×109
Blue-Whiet Screening:
Yes
Strain:
K12
Reduces Recombination:
Yes
Cloning Methylated DNA:
No
Improves Plasmid Quality:
Yes
Preparing Unmethylated DNA:
Not suitable
T1 Phage Resistant:
No
RecA Deficient:
Yes
Additional information
| OPTIONS | 20x50ul (20 tubes), >1×10⁹, 96x50ul (96 well PCR plate), >1×10⁹, One-year pre-payment: 144x10x100μl or 15 PCR plates (DA-196 Kit). Shipping and handling charges apply. >1×10⁹., 96 x 50µl (Bendable tear-off 96 well PCR plate) |
|---|
Manual & Protocols
COA ( Certificate of Analysis )
MSDS & Certificates
The Q&A for this product will be available soon.
