RNase I, E. coli
$0.00 – $537.00
SKU | Volumes | Concentration | Price | Quantity | ||
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RNIE-100 | 6,000 units | 24,000 U/ml | $179.00 | |||
RNIE-200 | 24,000 units | 24,000 U/ml | $537.00 | |||
RNIE-OEM | Any Size | Please inquire |
- Description
- Additional information
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Description
Description:
RNase I catalyzes the hydrolysis of single-stranded RNA to nucleoside 3′-monophosphates via 2′, 3′ cyclic monophosphate intermediates. Note: The enzyme is inactivated by heating at 70°C for 15 minutes, eliminating phenol extractions to remove the enzyme.
Applications:
- Degradation of single-stranded RNA to mono-, di- and trinucleotide
- Used in ribonuclease protection assays
Source:
An E. coli strain containing a genetic fusion of the RNase I gene (rna) from E. coli and the gene coding for maltose-binding protein (MBP)
Unit Definition:
One unit of enzyme required to catalyze the degradation of 100 ng of E. coli ribosomal RNA per second into acid-soluble nucleotides at 37°C.
Storage Buffer:
Supplied in 50% Glycerol, 50 mM Tris-HCl (pH 7.5), 100 mM NaCl, 0.01 mM EDTA.
Recommended Storage Condition: -20°C
Additional information
OPTIONS | 6,000 units (24,000 U/ml), 24,000 units (24,000 U/ml), Any Size |
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