RNase I, E. coli

$0.00$537.00

RNase I catalyzes the hydrolysis of single-stranded RNA to nucleoside 3’-monophosphates via 2’, 3’ cyclic monophosphate intermediates.
SKU: RNIE Categories: ,
SKU Volumes Concentration PriceQuantity
RNIE-100 6,000 units24,000 U/ml $179.00
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RNIE-200 24,000 units24,000 U/ml $537.00
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RNIE-OEM Any Size Please inquire
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Description

Description:
RNase I catalyzes the hydrolysis of single-stranded RNA to nucleoside 3′-monophosphates via 2′, 3′ cyclic monophosphate intermediates. Note: The enzyme is inactivated by heating at 70°C for 15 minutes, eliminating phenol extractions to remove the enzyme.

Applications:

  • Degradation of single-stranded RNA to mono-, di- and trinucleotide
  • Used in ribonuclease protection assays

Source:
An E. coli strain containing a genetic fusion of the RNase I gene (rna) from E. coli and the gene coding for maltose-binding protein (MBP)

Unit Definition:
One unit of enzyme required to catalyze the degradation of 100 ng of E. coli ribosomal RNA per second into acid-soluble nucleotides at 37°C.

Storage Buffer:
Supplied in 50% Glycerol, 50 mM Tris-HCl (pH 7.5), 100 mM NaCl, 0.01 mM EDTA.

Recommended Storage Condition: -20°C

Additional information

OPTIONS

6,000 units (24,000 U/ml), 24,000 units (24,000 U/ml), Any Size

Manual & Protocols

 

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