MC Cloning 5’/3’-RACE Kit

$0.00$680.00

The MC Cloning 5’/3′ RACE Kit provides a convenient method for performing both 5′ and 3′ rapid amplifications of cDNA ends (RACE) with optimized primers for cloning any gene of interest.

SKU: SKU49543 Categories: ,
SKU OPTIONS PriceQuantity
MCC5-100 10 reactions $453.00
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MCC5-200 20 reactions $680.00
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MCC5-OEM Any size Please inquire
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Description

Description:
The MC Cloning 5’/3′ RACE Kit provides a convenient method for performing both 5′ and 3′ rapid amplifications of cDNA ends (RACE) with optimized primers for cloning any gene of interest. MCLAB’s SmartRT reverse transcriptase is an engineered MMLV RT with increased thermostability, improved cDNA synthesis ability, and a reduced RNase H activity. The enzyme also has a terminal transferase activity. This tailing activity of the SmartRT reverse transcriptase allows to synthesize complete cDNA by SMART (Switching Mechanism At 5′ end of RNA Transcript) cDNA synthesis technology. With a few additional nucleotides added to the 3′ end of the first-strand cDNA, 5′ SMART universal oligo, containing terminal complementation to the nucleotides at 3′ end of the first-strand cDNA, can be annealed to first-strand cDNA tail and serves as an extended template for RT. The switch of template from mRNA to 5′ SMART universal oligo produces a complete cDNA copy of transcript RNA with 5′ SMART universal oligo at the end. The cDNA transcript from SmartRT can be used directly in 5′ and 3′ RACE with MCLAB’s I-5™ 2X High-Fidelity Master Mix.

Features:
• Specific full length cDNA enrichment technique
• Optimized primer set for full length cDNA sequencing
• Simplified protocol without 5′ end adaptor-ligation step

Applications:
• Generating full-length cDNAs
• Structural and expression studies of RNA molecules
• Isolation and characterization of 5′ or 3′ ends from low-copy RNA messages
• Amplification and further cloning of rare mRNAs
• Use in conjunction with exon-trapping methods

Components:
1.

Components Cap Color Volume (10 rxn) Volume (20 rxn)
Oligo dT Primer Orange 10 µl 20 µl
5X RT Reaction Buffer Green 40 µl 80 µl
dNTP (10 mM) Yellow 10 µl 20 µl
DTT (100 mM) Yellow 5 µl 10 µl
SmartRT™ Reverse Transcriptase Clear 20 µl 40 µl
5′ PCR Universal Primer (10µM) Red 10 µl 20 µl
3′ Universal Primer (10µM) Red 10 µl 20 µl
I-5™ 2X High-Fidelity Master Mix Purple 100 µl 200 µl
RNAse Inhibitor Blue 5 µl 10 µl

Storage Condition:
-20 °C

2. 5′ SMART Universal Oligo, 10 µl/20 µl. Storage Condition: -80 °C

Additional information

OPTIONS

10 reactions, 20 reactions, Any size

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