Inorganic Pyrophosphatase, E. coli

$0.00$453.20

Inorganic pyrophosphatase (PPase) is ubiquitous in nature and plays an important role in energy metabolism, providing a thermodynamic pull for biosynthetic reactions such as protein, RNA, and DNA synthesis.
SKU: IPE Categories: ,
SKU OPTIONS PriceQuantity
IPE-100 0.25 mg (1 mg/ml) $141.63
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IPE-200 1 mg (1 mg/ml) $453.20
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IPE-OEM Any Size Please inquire
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Description

Description:
Inorganic pyrophosphatase (PPase) is ubiquitous in nature and plays an important role in energy metabolism, providing a thermodynamic pull for biosynthetic reactions such as protein, RNA, and DNA synthesis. Escherichia coli K-12 gene ppa encoding inorganic pyrophosphatase (PPase) was cloned and sequenced. The 5′-end of the ppa mRNA was identified by primer extension mapping.

Source:
An E. coli strain carrying a plasmid encoding pyrophosphatase from E. coli K-12

Applications:

  • Role in protein, RNA, and DNA synthesis
  • Catalyzing the reaction PPi + H2O —> 2Pi

Unit Definition:
One unit will release 1.0 µmole of inorganic orthophosphate per minute at pH 8.5 at 37°C.

Specific Activity:  3,500 U/mg

Recommended Storage Condition: -20°C

Reference:
Kornberg, A. 1962. On the metabolic significance of phosphorolytic and pyrophosphorolytic reactions, p. 251-264. In H. Kasha and P. Pullman (ed.), Horizons in biochemistry. Academic Press, New York.

Additional information

OPTIONS

0.25 mg (1 mg/ml), 1 mg (1 mg/ml), Any Size

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