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Endo S2

Placeholder Endo S

$0.00 – $840.00

MCLAB’s Endo S2 enzyme is a recombinant endo-β-N-acetylglucosaminidase cloned from Streptococcus pyogenes and expressed in E. coli. This highly specific IgG deglycosylation enzyme hydrolyzes the His-tag core (β-1,4 linkage between two N-acetylglucosamines) of Fc N-linked glycans.

SKU: N/A Categories: ,
SKU Options PriceQuantity
ENDOS2-100 Endo S2 (Glycerol-free) (6,000 U) $210.00
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ENDOS2-200 Endo S2 (Glycerol-free) (30,000 U) $840.00
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ENDOS2-OEM Endo S2 (Glycerol-free) (Any Size) Please inquire
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ENDOS2-100G Endo S2 (10% Glycerol) (6,000 U) $210.00
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ENDOS2-200G Endo S2 (10% Glycerol) (30,000 U) $840.00
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ENDOS2-OEMG Endo S2 (10% Glycerol) (Any Size) Please inquire
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Description

Endo S2

• Glycerol-free: Cat# ENDOS2-100, ENDOS2-200, ENDOS2-OEM
• 10% glycerol: Cat# ENDOS2-100G, ENDOS2-200G, ENDOS2-OEMG

Description:

MCLAB’s Endo S2 enzyme is a recombinant endo-β-N-acetylglucosaminidase cloned from Streptococcus pyogenes and expressed in E. coli. This highly specific IgG deglycosylation enzyme hydrolyzes the His-tag core (β-1,4 linkage between two N-acetylglucosamines) of Fc N-linked glycans.

Endo S2 efficiently cleaves:

  • High-mannose N-glycans
  • Hybrid N-glycans
  • Bi-antennary complex N-glycans
  • Glycans with or without core fucosylation

The enzyme is widely used in antibody glycosylation analysis, Fc engineering, glycoform remodeling, and biotherapeutic characterization workflows. The enzyme is supplied glycerol-free, making it well suited for HPLC, LC-MS, and other mass spectrometry-intensive workflows.

The enzyme with glycerol as protectant is also available.

Applications:

  • IgG Fc deglycosylation
  • Antibody glycoengineering
  • Glycan remodeling and transfer (oxazoline forms)
  • Biotherapeutic development
  • Mass spectrometry glycan profiling
  • SDS-PAGE mobility shift analysis

Source:

Endo S2 is cloned from Streptococcus pyogenes and recombinantly expressed in E. coli as a fusion protein with a His-tag.

Standard Protocol (Reference)

  • Dissolve 1–20 μg glycoprotein in deionized water.
  • Add 1 μL 500 mM Tris-HCl (pH 7.5).
  • Adjust total volume to 10 μL.
  • Add 1–2 μL Endo S2.
  • Incubate at 37°C for 60–120 minutes.
  • Analyze via SDS-PAGE or mass spectrometry.

Most neutral biological buffers (Tris, PBS) are suitable. Optimization may be required outside neutral pH.

Unit Definition (Standard Assay):

One unit deglycosylates ≥95% of 1 μg human IgG in:
10 mM sodium phosphate
150 mM NaCl
pH 7.4
37°C for 30 minutes

Alternative Activity Definition:

One unit removes >95% carbohydrate from 5 μg human IgG in 1 hour at 37°C (10 μL reaction).
Specific Activity: ≥200,000 U/mL

Additional Activity Measurement:

50% digestion of Cy5-labeled Glycan G2 (0.2 pmol) using 0.2 μg recombinant Endo S2 under assay conditions.

Reagents Supplied with:

Each product configuration includes:

  • Endo S2 enzyme (His-tagged)
  • 10X Reaction Buffer

Enzyme Concentration: 200,000 units/mL

Storage Buffer for Endo S2 (Glycerol Free) Enzyme

20 mM Tris-HCl
50 mM NaCl
1 mM EDTA
pH 7.5 (25°C)

Storage Buffer for Endo S2 (10% Glycerol) Enzyme

20 mM Tris-HCl
50 mM NaCl
1 mM EDTA
pH 7.5 (25°C)
10% Glycerol

Molecular Weight

Apparent molecular weight: 92 kDa

Related Terms: Endo S2 enzyme, Endo-β-N-acetylglucosaminidase S2, IgG deglycosylation enzyme, Fc glycan removal enzyme, Antibody glycoengineering enzyme, Recombinant Endo S2 protein

Recommended Storage Condition: -20°C, avoid repeated free-thaw

Endo S vs. Endo S2

ATTRIBUTE Endo S Endo S2
Enzyme Type IgG-specific endoglycosidase Endo-β-N-acetylglucosaminidase S2
Primary Substrate Native IgG Fc N-glycans IgG Fc N-glycans + α1-acid glycoprotein
Substrate Breadth IgG-specific Broader (IgG + non-IgG glycoproteins)
Cleavage Site β-1,4 linkage in chitobiose core of heavy chain β-1,4 linkage between GlcNAc residues in Fc N-glycans
His-Tag (Removable) YES — Ni-NTA YES — Ni-NTA
Specific Activity Per unit: >95% removal from 5 μg IgG in 1 hr at 37°C Stock ≥200,000 U/mL — 1 unit deglycosylates ≥95% of 1 μg IgG in 30 min at 37°C
Core Fucosylation Activity Active (with or without) Active
Bisecting GlcNAc Activity Active (with or without) Active
Glycerol-Free SKUs ENDOS-100, ENDOS-200, ENDOS-OEM ENDOS2-100, ENDOS2-200, ENDOS2-OEM
10% Glycerol SKUs ENDOS-100G, ENDOS-200G, ENDOS-OEMG ENDOS2-100G, ENDOS2-200G, ENDOS2-OEMG
OEM Quantities Available Available
Expression Host E. coli (recombinant) E. coli (recombinant)
Cloned From Streptococcus pyogenes Streptococcus pyogenes
Storage -20°C, avoid repeated freeze-thaw -20°C, avoid repeated freeze-thaw
Best For Standard IgG Fc glycan removal with clean enzyme removal post-reaction Broader N-glycan analysis, LC-MS, HPLC, ADC synthesis, biotherapeutic characterization
Recommended When Working with native IgG and need clean, removable enzyme Need broader substrate coverage, HPLC/MS-ready workflow or glycerol-free format

For Research Use Only

 

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