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Endo S

MC Prepaid Certificate
Placeholder Endo S2

$0.00 – $945.00

 Buy recombinant Endo S for IgG Fc deglycosylation and antibody glycan analysis. High purity, MS-compatible, research grade enzyme.

SKU: N/A Categories: ,
SKU Options PriceQuantity
ENDOS-100 Endo S (Glycerol-free) (18,000 U) $238.00
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ENDOS-200 Endo S (Glycerol-free) (90,000 U) $945.00
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ENDOS-OEM Endo S (Glycerol-free) (Any Size) Please inquire
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ENDOS-100G Endo S (10% Glycerol) (18,000 U) $238.00
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ENDOS-200G Endo S (10% Glycerol) (90,000 U) $945.00
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ENDOS-OEMG Endo S (10% Glycerol) (Any Size) Please inquire
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Bulk Orders Add selected to cart ✓

Description

Endo S

• Glycerol-free: Cat# ENDOS-100, ENDOS-200, ENDOS-OEM
• 10% glycerol: Cat# ENDOS-100G, ENDOS-200G, ENDOS-OEMG

From left to right: deglycosylated ThT + Endo S, deglycosylated ThT, deglycosylated ThT + biotinylated Endo S. SDS-PAGE shows Mw of deglycosylated ThT decreases slightly after incubation with Endo S or biotinylated Endo S confirming activity of Endo S and biotinylated Endo S.

Description:

IgG-Specific Endoglycosidase with His-tag.

Endo S is a highly specific endoglycosidase that selectively removes N-linked glycans from the chitobiose core of the heavy chain of native IgG. The enzyme hydrolyzes the β-1,4 linkage between the two N-acetylglucosamine residues within Fc N-glycans.

MCLAB’s Endo S is engineered with a His-tag, allowing rapid and efficient removal of the enzyme from deglycosylation reactions using Ni-NTA-based affinity matrices. The enzyme is supplied glycerol-free, making it well suited for HPLC, LC-MS, and other mass spectrometry-intensive workflows.

The enzyme with glycerol as protectant is also available.

Specificity

Endo S exhibits high specificity toward native IgG Fc N-glycans.
Substrate Requirements:

  • Active on substrates with or without core α(1-6) fucosylation
  • Active on substrates with or without bisecting N-acetylglucosamine
  • Does not require a strict peptide sequence for activity

Not Active On:

  • Triantennary N-glycans
  • Tetraantennary N-glycans
  • Highly sialylated complex glycans

Substrate format may include protein, peptide, asparagine-linked glycan, or free glycan.

Applications:

  • IgG deglycosylation
  • Biotherapeutic characterization
  • Antibody structure-function studies
  • Glycan sequencing
  • Proteomics
  • Recombinant glycoprotein analysis
  • Expression system validation

Source:

Endo S is cloned from Streptococcus pyogenes and recombinantly expressed in E. coli as a fusion protein with a His-tag.

Recommended Reaction Conditions

Final Reaction Buffer (1X):

  • 5 mM CaCl₂
  • 50 mM sodium acetate
  • pH 5.5 (25°C)

Incubate at 37°C.

Unit Definition (Standard Assay):

One unit is defined as the amount of enzyme required to remove >95% of carbohydrate from 5 μg of native mouse monoclonal IgG in 1 hour at 37°C in a 10 μL total reaction volume.

Unit Assay Conditions

5 μg IgG in 1X reaction buffer is incubated with serial dilutions of Endo S for 1 hour at 37°C. Reaction products are analyzed by SDS-PAGE.

Reagents Supplied with:

Each product configuration includes:

  • Endo S enzyme (His-tagged)
  • 10X Reaction Buffer

Enzyme Concentration: 200,000 units/mL

Storage Buffer for Endo S (Glycerol Free) Enzyme

20 mM Tris-HCl
50 mM NaCl
1 mM EDTA
pH 7.5 (25°C)

Storage Buffer for Endo S (10% Glycerol) Enzyme

20 mM Tris-HCl
50 mM NaCl
1 mM EDTA
pH 7.5 (25°C)
10% Glycerol

Molecular Weight

Apparent molecular weight: 136 kDa (His-tagged fusion protein)

Heat Inactivation

55°C for 10 minutes

Enzyme Removal

The His-tag enables affinity-based removal using Ni-NTA agarose beads.
* Binding capacity: 1 mg His-tagged protein per mL of Ni-NTA agarose beads
* Removal can be linearly scaled with reaction volume
This feature makes Endo S ideal for workflows requiring clean downstream MS or HPLC analysis.

Related Terms: Endo S2 enzyme, Endo-β-N-acetylglucosaminidase S2, IgG deglycosylation enzyme, Fc glycan removal enzyme, Antibody glycoengineering enzyme, Recombinant Endo S2 protein

Recommended Storage Condition: -20°C, avoid repeated free-thaw

Endo S vs. Endo S2

ATTRIBUTE Endo S Endo S2
Enzyme Type IgG-specific endoglycosidase Endo-β-N-acetylglucosaminidase S2
Primary Substrate Native IgG Fc N-glycans IgG Fc N-glycans + α1-acid glycoprotein
Substrate Breadth IgG-specific Broader (IgG + non-IgG glycoproteins)
Cleavage Site β-1,4 linkage in chitobiose core of heavy chain β-1,4 linkage between GlcNAc residues in Fc N-glycans
His-Tag (Removable) YES — Ni-NTA YES — Ni-NTA
Specific Activity Per unit: >95% removal from 5 μg IgG in 1 hr at 37°C Stock ≥200,000 U/mL — 1 unit deglycosylates ≥95% of 1 μg IgG in 30 min at 37°C
Core Fucosylation Activity Active (with or without) Active
Bisecting GlcNAc Activity Active (with or without) Active
Glycerol-Free SKUs ENDOS-100, ENDOS-200, ENDOS-OEM ENDOS2-100, ENDOS2-200, ENDOS2-OEM
10% Glycerol SKUs ENDOS-100G, ENDOS-200G, ENDOS-OEMG ENDOS2-100G, ENDOS2-200G, ENDOS2-OEMG
OEM Quantities Available Available
Expression Host E. coli (recombinant) E. coli (recombinant)
Cloned From Streptococcus pyogenes Streptococcus pyogenes
Storage -20°C, avoid repeated freeze-thaw -20°C, avoid repeated freeze-thaw
Best For Standard IgG Fc glycan removal with clean enzyme removal post-reaction Broader N-glycan analysis, LC-MS, HPLC, ADC synthesis, biotherapeutic characterization
Recommended When Working with native IgG and need clean, removable enzyme Need broader substrate coverage, HPLC/MS-ready workflow or glycerol-free format

For Research Use Only

Additional information

Options

Endo S (Glycerol-free) (18,000 U), Endo S (Glycerol-free) (90,000 U), Endo S (Glycerol-free) (Any Size), Endo S (10% Glycerol) (18,000 U), Endo S (10% Glycerol) (90,000 U), Endo S (10% Glycerol) (Any Size)

 

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