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Molecular Cloning, Reverse Transcriptase and RNA Polymerases, RT-PCR KitsT7 High Yield In Vitro Transcription Kit
Phage RNA polymerases are widely used for in vitro synthesis of RNA transcripts from DNA templates, which have a double-stranded promoter (at least 19 bases) upstream of the sequence to be…
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Enzymes, Molecular Cloning, Reverse Transcriptase and RNA Polymerases, RT-PCR KitsT7 RNA Polymerase
T7 RNA polymerase is a DNA-dependent RNA polymerase that catalyzes the formation of RNA in the 5′→ 3′ direction.
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Culture Plates, Molecular Cloning
Terrific Broth Agar Plates
1.2% Bacto tryptone, 2.4% yeast extract, 1.6% glycerol, 1.5% agar, 72mM K2HPO4, and 17mM KH2PO4.
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Culture Plates, Molecular Cloning
YT Agar Plates
1X YT Agar Plate: 0.8% Tryptone, 0.5% yeast extract, 0.5% NaCl, 1.5% agar
2X YT Agar Plate: 1.6% Tryptone, 1.0% yeast extract, 0.5% NaCl, 1.5% agar
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