I-5™ 2X Hi-Fi PCR Master Mix

$0.00$642.00

I-5 High-Fidelity DNA Polymerase is an ultra-high fidelity and high processivity enzyme. It produces the most accurate copies of DNA, and performs at an ultra-high rate. I-5 is perfect for applications such as cloning amplified material for protein expression in vitro, SNP analysis by sequencing, and high-specificity PCR.

Please note: 2X Hi-Fi HTP PCR Master Mix has been replaced with I-5™ 2X Hi-Fi PCR Master Mix for enhanced fidelity.

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SKU OPTIONS PriceQuantity
I5HM-100 100 reactions (50 ul vol), 2 x 1.25mL $159.00
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I5HM-200 500 reactions (50 ul vol), 12.5 mL $642.00
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I5HM-5000 5,000 reactions (50 ul vol) Please inquire
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I5HM-25k 25,000 reactions (50 ul vol) Please inquire
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I5HM-OEM OEM Please inquire
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Description

Description:
I-5 High-Fidelity DNA Polymerase (pfu fusion) is an ultra-high fidelity and high processivity enzyme. It produces the most accurate copies of DNA, and performs at an ultra-high rate. I-5 is perfect for applications such as cloning amplified material for protein expression in vitro, SNP analysis by sequencing, and high specificity PCR.

Convenient Usage:
I-5 2X High-Fidelity Master Mix has a buffer system that is designed to offer maximum performance of the I-5 High-Fidelity DNA polymerase.  It offers the highest fidelity and incredibly robust performance. The convenient master mix allows the reaction to be set up at room temperature and only requires the addition of primers and DNA template for the amplification.

Features:

  • Ultra-High Fidelity: >100X higher than Taq
  • High Speed: 20X faster than Pfu
  • High Yield: Increased product yield using minimal amount of enzyme
  • Versatile: Can be used for routine PCR as well as long or difficult templates
  • Easy Format: Requires the addition of PCR primers and DNA template for amplification

Applications:

  • High-fidelity PCR amplification
  • High-throughput PCR
  • Various cloning technologies
  • Long or difficult amplifications

Recommended Storage Condition: -20°C

Additional information

OPTIONS

100 reactions (50 ul vol), 2 x 1.25mL, 500 reactions (50 ul vol), 12.5 mL, 5,000 reactions (50 ul vol), 25,000 reactions (50 ul vol), OEM

The Q&A for this product will be available soon.