Sample Preparation

Sample Preparation

Learn about the newly introduced MC Easy Format™ sample submission method for you to save time and money.

 

For separated DNA templates and primers, detailed requirements are shown below:

Template Type DNA Concentration DNA Volume Primer Concentration Primer Volume
Plasmid 100 ng/µl 10 µl 3.2 µM 10 µl
PCR (100-500bp) 5-10 ng/µl 10 µl 3.2 µM 10 µl
PCR(500bp-1kb) 10-20 ng/µl 10 µl 3.2 µM 10 µl
PCR(>1kb) 20-40 ng/µl 10 µl 3.2 µM 10 µl
Cosmid 0.5-1 µg/µl 20 µl 3.2 µM 10 µl
Fosmid 0.5-1 µg/µl 20 µl 3.2 µM 10 µl

 

For mixed DNA templates and primers, detailed requirements are shown below:

Template Type Amount of DNA Amount of Primer Volume (DNA+Primer)
Plasmid 500 ng 9 pmol 15 µl
PCR (100-500bp) 20 ng 9 pmol 15 µl
PCR (500bp-1kb) 30 ng 9 pmol 15 µl
PCR (>1kb) 50 ng 9 pmol 15 µl