Glutathione Agarose for GST-tagged Cartridges
$112.17 – $452.07
Glutathione S-transferase (GST) gene fusion systems have been widely used for obtaining large amounts of desirable protein in Escherichia coli.
| SKU | OPTIONS | Price | Quantity | ||
|---|---|---|---|---|---|
| GAB-CC1x5 | 5 x 1ml Glutathione Agarose chromatography cartridge | $134.83 | |||
| GAB-CC5x1 | 1 x 5ml Glutathione Agarose chromatography cartridge | $112.17 | |||
| GAB-CC5x5 | 5 x 5ml Glutathione Agarose chromatography cartridge | $452.07 |
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Description
Description
Glutathione Agarose CL-6B is based on highly cross-linked agarose 6% beads which have structural characteristics that result in superb physical strength, excellent flow properties, low backpressure, and an open pore structure. Glutathione Agarose CL-6B allows operation at very high flow rates, resulting in increased throughput and rapid column cleaning between chromatography runs.
Specifications
| Chromatography technique | GST-tagged protein purification |
| Matrix | Highly cross-linked 6% beaded agarose |
| Active group | Glutathione |
| Active group density | >40 umol/ml drained medium |
| Spacer | 1,4-bis(2,3-epoxypropoxy)butane (12 atom stable and uncharged ether hydrophilic linkage) |
| Bead geometry & size | Spherical 50 to 150 um |
| Bead mean diameter d50v | 90 um |
| Linear flow velocity | <75 cm/h at 25 degC, HR 16/10 column, 5 cm bed height |
| Recommended linear flow rate | <30 cm/h |
| Pressure/flow specification | Base matrix 100-200 cm/h, pressure drop cm H2O/bed height = 15, bed height 10 cm, 5 cm i.d. |
| 1ml Cartridge bed dimensions | 7×25 mm |
| 1ml Cartridge bed height | 25 mm |
| 1ml Cartridge bed volume | 1 ml |
| 1ml Cartridge column I.D | 7 mm |
| 5ml Cartridge bed dimensions | 16×25 mm |
| 5ml Cartridge bed height | 25 mm |
| 5ml Cartridge bed volume | 5 ml |
| 5ml Cartridge column I.D | 16 mm |
| Maximum cartridge flow rate | 5 ml/min (1 ml cartridge) or 20 ml/min (5ml cartridge) |
| Recommended cartridge flow rate | 1 ml/min (1 ml cartridge) or 5 ml/min (5 ml cartridge) |
| Maximum pressure during operation | 5 bar [0.5 MPa] (70 psi) |
| pH Stability working range | 3 to 13 |
| pH Stability cleaning in place (cip) | 2 to 14 |
| Chemical stability | Stable to commonly used aqueous solutions. Can be used with non-ionic detergents, denaturing solvents, e.g. 8 M urea and 6 M guanidine hydrochloride, Stable in organic solvents, e.g. 50% methylformamide and 50% dioxane. |
| Storage | 2 to 8 degC |
Additional information
| OPTIONS | 5 x 1ml Glutathione Agarose chromatography cartridge, 1 x 5ml Glutathione Agarose chromatography cartridge, 5 x 5ml Glutathione Agarose chromatography cartridge |
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