Dye Terminator 5X Sequencing Buffer

$0.00$3,959.84

The Dye Terminator 5X Sequencing Buffer reduces sequencing costs without affecting sequence accuracy or reads length. Its buffer system enables to use of less dye terminator mix.
SKU OPTIONS PriceQuantity
SBUF-100 1 ml $28.33
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SBUF-110 28 ml $532.51
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SBUF-120 233 ml $3,959.84
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SBUF-OEM Any size Please inquire
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Description

Description:
The Dye Terminator 5X Sequencing Buffer reduces sequencing costs without affecting sequence accuracy or reads length. Its buffer system enables to use of less dye terminator mix.

Applications:

  • DNA sequencing
  • Fragment analysis

Recommended Storage Condition: 4°C

Recommended Protocol:
Prepare sequencing reactions (10µl rxn) according to the following:
– Add dye terminator mix 0.25-0.5µl
– Add 5X Sequencing Buffer 2µl
– Add template (100-200 ng/µl) 1 µl
– Add primer (3 pmol/µl) 1 µl
– Add H2O to 10 µl
Cycle the reaction according to the following protocol:

Step 1: 96°C  3 minutes
Step 2: 96°C  10 seconds
Step 3: 50°C  5 seconds
Step 4: 60°C  2 minutes and 30 seconds
(25 to 30 cycles from step 2 to step 4)
Step 5: 4°C hold

Notes:
Other cycle conditions may work well for individual users. These conditions work well for most of the samples processed in our lab using reduced volume and reduced terminator mix concentrations.

Additional information

OPTIONS

1 ml, 28 ml, 233 ml, Any size

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