Dye Terminator 5X Sequencing Buffer
$0.00 – $3,959.84
| SKU | OPTIONS | Price | Quantity | ||
|---|---|---|---|---|---|
| SBUF-100 | 1 ml | $28.33 | |||
| SBUF-110 | 28 ml | $532.51 | |||
| SBUF-120 | 233 ml | $3,959.84 | |||
| SBUF-OEM | Any size | Please inquire |
- Description
- Additional information
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Description
Description:
The Dye Terminator 5X Sequencing Buffer reduces sequencing costs without affecting sequence accuracy or reads length. Its buffer system enables to use of less dye terminator mix.
Applications:
- DNA sequencing
- Fragment analysis
Recommended Storage Condition: 4°C
Recommended Protocol:
Prepare sequencing reactions (10µl rxn) according to the following:
- Add dye terminator mix 0.25-0.5µl
- Add 5X Sequencing Buffer 2µl
- Add template (100-200 ng/µl) 1 µl
- Add primer (3 pmol/µl) 1 µl
- Add H2O to 10 µl
Cycle the reaction according to the following protocol:
Step 1: 96°C 3 minutes
Step 2: 96°C 10 seconds
Step 3: 50°C 5 seconds
Step 4: 60°C 2 minutes and 30 seconds
(25 to 30 cycles from step 2 to step 4)
Step 5: 4°C hold
Notes:
Other cycle conditions may work well for individual users. These conditions work well for most of the samples processed in our lab using reduced volume and reduced terminator mix concentrations.
Additional information
| OPTIONS | 1 ml, 28 ml, 233 ml, Any size |
|---|
COA ( Certificate of Analysis )
MSDS & Certificates
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