Blunting Express Kit
$85.00 – $340.00
The Blunting Express Kit can be used to generate blunt-ended DNA fragments for subsequent use in ligation, cloning, cDNA construction, and MCLAB’s Topomize Amplicon Library Prep Kit (Cat# TopoA-50A or TopoA-50B). Our proprietary enzyme mix effectively fills in 5’ overhangs and eliminates A-tailed 3’ overhangs on the template.
| SKU | OPTIONS | Price | Quantity | ||
|---|---|---|---|---|---|
| BLEK-100 | 20 rxn | $85.00 | |||
| BLEK-200 | 50 rxn | $198.00 | |||
| BLEK-300 | 100 rxn | $340.00 |
- Description
- Additional information
- Documents
Description
Description:
The Blunting Express Kit can be used to generate blunt-ended DNA fragments for subsequent use in ligation, cloning, cDNA construction, and MCLAB’s Topomize Amplicon Library Prep Kit (Cat# TopoA-50A or TopoA-50B). Our proprietary enzyme mix effectively fills in 5’ overhangs and eliminates A-tailed 3’ overhangs on the template. This Blunting Express Kit is optimized to provide a rapid workflow with only 2 minutes of incubation. Efficiency is combined with a high performance to streamline your blunt-end objectives.
Features:
Due to our proprietary Enzyme Mix, this kit provides a rapid workflow with a blunting step that requires only two minute incubation. Its high efficiency is combined with a high performance to streamline the blunt end cloning objectives.
Recommended Storage Condition: -20°C
Kit Components:
- 10x Buffer
- dNTP Mix (25mM)
- Enzyme Mix
Figure:
PCR fragment size checked before and after end blunting by the kit. Data are generated by Applied Biosystems 3730/3730xl DNA Analyzer.
Additional information
| OPTIONS | 20 rxn, 50 rxn, 100 rxn |
|---|