5’ Adenylation (rApp Modification) Service

$453.00$5,665.00

The custom 5’ adenylation service is designed for generating 5’ pre-adenylated DNA/RNA oligonucleotides. Adenylated oligos with a pyrophosphate linkage are substrates of T4 RNA ligase in the absence of ATP, which can significantly reduce undesired self-ligation and other side products.

SKU: 5APP Categories: ,
SKU OPTIONS PriceQuantity
5APP-100 100 nmole DNA Oligo $453.00
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5APP-200 250 nmole DNA Oligo $566.00
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5APP-300 1 µmole DNA Oligo $1,133.00
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5APP-400 5 µmole DNA Oligo $4,532.00
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5APP-500 10 µmole DNA Oligo $5,665.00
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Description

Description:
The custom 5’ adenylation service is designed for generating 5’ pre-adenylated DNA/RNA oligonucleotides. Adenylated oligos with a pyrophosphate linkage are substrates of T4 RNA ligase in the absence of ATP, which can significantly reduce undesired self-ligation and other side products.

Application:
5’ adenylated oligos are used in sequence detection, sequence capture, sequencing by ligation and attachment of adapters (e.g. RNA library preparation for NGS and miRNA library construction), etc.

Starting material requirement:
1)    Order oligos with 5’ phosphate.
2)    To prevent a 5’ adenylated oligo from self-ligating, a blocking group is required on the 3’ end, such as a 2’, 3’-Dideoxynucleotide or 3’ (C3) Propyl Spacer.
3)    5’ adenylated oligos require an extra purification after the adenylation step and are not compatible with heat-sensitive modifications. Prices are valid for oligonucleotides up to 30 bases. For longer oligos, please inquire for a custom quotation.
Adenylation

Additional information

OPTIONS

100 nmole DNA Oligo, 250 nmole DNA Oligo, 1 µmole DNA Oligo, 5 µmole DNA Oligo, 10 µmole DNA Oligo

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