5’ Adenylation (rApp Modification) Service
$453.00 – $5,665.00
The custom 5’ adenylation service is designed for generating 5’ pre-adenylated DNA/RNA oligonucleotides. Adenylated oligos with a pyrophosphate linkage are substrates of T4 RNA ligase in the absence of ATP, which can significantly reduce undesired self-ligation and other side products.
| SKU | OPTIONS | Price | Quantity | ||
|---|---|---|---|---|---|
| 5APP-100 | 100 nmole DNA Oligo | $453.00 | |||
| 5APP-200 | 250 nmole DNA Oligo | $566.00 | |||
| 5APP-300 | 1 µmole DNA Oligo | $1,133.00 | |||
| 5APP-400 | 5 µmole DNA Oligo | $4,532.00 | |||
| 5APP-500 | 10 µmole DNA Oligo | $5,665.00 |
- Description
- Additional information
- Documents
- Q&A
Description
Description:
The custom 5’ adenylation service is designed for generating 5’ pre-adenylated DNA/RNA oligonucleotides. Adenylated oligos with a pyrophosphate linkage are substrates of T4 RNA ligase in the absence of ATP, which can significantly reduce undesired self-ligation and other side products.
Application:
5’ adenylated oligos are used in sequence detection, sequence capture, sequencing by ligation and attachment of adapters (e.g. RNA library preparation for NGS and miRNA library construction), etc.
Starting material requirement:
1) Order oligos with 5’ phosphate.
2) To prevent a 5’ adenylated oligo from self-ligating, a blocking group is required on the 3’ end, such as a 2’, 3’-Dideoxynucleotide or 3’ (C3) Propyl Spacer.
3) 5’ adenylated oligos require an extra purification after the adenylation step and are not compatible with heat-sensitive modifications. Prices are valid for oligonucleotides up to 30 bases. For longer oligos, please inquire for a custom quotation.
Additional information
| OPTIONS | 100 nmole DNA Oligo, 250 nmole DNA Oligo, 1 µmole DNA Oligo, 5 µmole DNA Oligo, 10 µmole DNA Oligo |
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